Analysis of the Humoral Immunal Response Transcriptome of Ectropis obliqua Infected by Beauveria bassiana.
Analysis of the Humoral Immunal Response Transcriptome of Ectropis obliqua Infected by Beauveria bassiana.
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白僵菌侵染斜生植物体液免疫反应转录组分析
DOI：
10.3390/insects13030225
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发表时间：
2022-02-24
期刊：
影响因子：
3
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Long Y;Gao T;Liu S;Zhang Y;Li X;Zhou L;Su Q;Xu L;Yang Y
茶尺蠖是我国茶园广泛分布的一种破坏性食叶害虫。这种害虫对多种杀虫剂有很强的抗药性。球孢白僵菌作为一种环境友好型昆虫病原菌，已被广泛应用于农业害虫防治。然而，B. bassiana对E.目前尚不清楚。本研究首次分离鉴定了一株高毒力B。球孢菌株利用转录组技术分析了大肠杆菌脂肪体和血细胞中免疫基因表达水平的差异。感染了B的大肠杆菌。bassiana，这提供了昆虫-病原体相互作用的分子见解。茶尺蠖是我国茶园的一种毁灭性咀嚼害虫。球孢白僵菌作为微生物杀虫剂能有效地防治E.然而，这种昆虫感染B. Bassiana在很大程度上未知。这里，在分离出一种高毒力的B. bassiana和E. Escherichia）的不同组织（包括血细胞和脂肪体）中基因表达的变化。使用转录组测序研究了被昆虫病原体感染的幼虫。在血细胞和脂肪体中共鉴定出5877个差异表达基因（DEG），其中5826个在血细胞中表达上调，5784个在脂肪体中表达上调。我们鉴定了249个免疫相关基因，包括模式识别受体、免疫效应子、信号调节子和免疫通路成员。定量实时PCR分析证实，几种模式识别受体上调血细胞和脂肪体，但其他下调。研究的免疫效应物（ATT和PPO-1）被抑制。结果表明，免疫基因的表达存在组织差异。本研究提供了大量的E.感染B后，为进一步了解E. A防御B。巴西纳。
Ectropis obliqua is a destructive leaf-eating pest that is widely distributed in China’s tea gardens. This pest shows remarkable resistance against multiple insecticides. As an environmentally friendly entomopathogen, Beauveria bassiana has been widely used to prevent agricultural pest infestations. However, the molecular mechanism of B. bassiana against E. obliqua remains unclear. We firstly isolated and identified a highly virulent B. bassiana strain. Using a transcriptome, we analyzed the differences of immune gene expression levels in fat bodies and hemocytes of E. obliqua that were infected by the B. bassiana, which provide molecular insights into the insect–pathogen interaction. Ectropis obliqua is a destructive masticatory pest in China’s tea gardens. Beauveria bassiana as microbial insecticides can effectively control E. obliqua larvae; however, the immune response of this insect infected by B. bassiana are largely unknown. Here, after isolating a highly virulent strain of B. bassiana from E. obliqua, the changes in gene expression among different tissues, including hemocytes and fat bodies, of E. obliqua larvae infected by the entomopathogen were investigated using transcriptome sequencing. A total of 5877 co-expressed differentially expressed genes (DEGs) were identified in hemocytes and fat bodies, of which 5826 were up-regulated in hemocytes and 5784 were up-regulated in fat bodies. We identified 249 immunity-related genes, including pattern recognition receptors, immune effectors, signal modulators, and members of immune pathways. A quantitative real-time PCR analysis confirmed that several pattern recognition receptors were upregulated in hemocytes and fat bodies; however, others were downregulated. The investigated immune effectors (ATT and PPO-1) were suppressed. The results showed that there were tissue differences in the expression of immune genes. This study provides a large number of immunity-related gene sequences from E. obliqua after being infected by B. bassiana, furthering the understanding of the molecular mechanisms of E. obliqua defenses against B. bassiana.
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影响因子：
3.2
作者：
Dang K;Doggett SL;Veera Singham G;Lee CY
通讯作者：
Lee CY
影响因子：
2.7
作者：
Kuzhuppillymyal-Prabhakarankutty, Laiju;Ferrara-Rivero, Fernando H.;Ek-Ramos, Maria Julissa
通讯作者：
Ek-Ramos, Maria Julissa
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
DOI：
10.1186/s43141-021-00227-x
发表时间：
2021-08-26
期刊：
Journal, genetic engineering & biotechnology
影响因子：
--
作者：
Aynalem B;Muleta D;Venegas J;Assefa F
通讯作者：
Assefa F
影响因子：
--
作者：
Aoki, Kiyoko F;Kanehisa, Minoru
通讯作者：
Kanehisa, Minoru