The sea lamprey Petromyzon marinus genome reveals the early origin of several chemosensory receptor families in the vertebrate lineage.
The sea lamprey Petromyzon marinus genome reveals the early origin of several chemosensory receptor families in the vertebrate lineage.
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DOI：
10.1186/1471-2148-9-180
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发表时间：
2009-07-31
影响因子：
3.4
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Libants S;Carr K;Wu H;Teeter JH;Chung-Davidson YW;Zhang Z;Wilkerson C;Li W
在有颌动物中，嗅觉上皮中表达的化学感受受体（CR）由编码气味受体（OR）、痕量胺相关受体（TAAR）、V1Rs和V2Rs的进化动态基因家族编码。在无脊椎脊索动物基因组中发现了有限数量的OR样序列。这些基因家族是否出现在基础或先进的脊椎动物尚未解决，因为这些家庭还没有被系统地检查无颌类基因组。Petromyzon是唯一现存的无颌脊椎动物，其基因组已被测序。七鳃鳗对几种气味非常敏感，但它们能检测到的氨基酸和类固醇比硬骨鱼少。这种可检测的气味物的减少的数量指示在海七鳃鳗中CR基因家族的减少的数量或CR家族内的基因的减少的数量，或两者。在七鳃鳗基因组中，我们确定了59个完整的单外显子CR基因，包括27个OR，28个TAAR和4个V1R样基因。这三个CR家族在寄生期和成虫期的嗅觉器官中都有表达。在七鳃鳗基因组中的广泛搜索未能确定在硬骨鱼基因组中极大扩展的多外显子V2R家族的潜在直系同源物或假基因，但确实发现了完整的钙敏感受体（CASR）和完整的代谢型谷氨酸受体（MGR）。我们的结论是，OR和V1R出现在脊索动物的头索动物，尾索动物分裂后，但在有颌和无颌脊椎动物的多样化。来自谷氨酸受体家族G蛋白偶联受体（最有可能是CASR）的V2R基因的出现和多样化发生在无颌分歧之后。
In gnathostomes, chemosensory receptors (CR) expressed in olfactory epithelia are encoded by evolutionarily dynamic gene families encoding odorant receptors (OR), trace amine-associated receptors (TAAR), V1Rs and V2Rs. A limited number of OR-like sequences have been found in invertebrate chordate genomes. Whether these gene families arose in basal or advanced vertebrates has not been resolved because these families have not been examined systematically in agnathan genomes. Petromyzon is the only extant jawless vertebrate whose genome has been sequenced. Known to be exquisitely sensitive to several classes of odorants, lampreys detect fewer amino acids and steroids than teleosts. This reduced number of detectable odorants is indicative of reduced numbers of CR gene families or a reduced number of genes within CR families, or both, in the sea lamprey. In the lamprey genome we identified a repertoire of 59 intact single-exon CR genes, including 27 OR, 28 TAAR, and four V1R-like genes. These three CR families were expressed in the olfactory organ of both parasitic and adult life stages. An extensive search in the lamprey genome failed to identify potential orthologs or pseudogenes of the multi-exon V2R family that is greatly expanded in teleost genomes, but did find intact calcium-sensing receptors (CASR) and intact metabotropic glutamate receptors (MGR). We conclude that OR and V1R arose in chordates after the cephalochordate-urochordate split, but before the diversification of jawed and jawless vertebrates. The advent and diversification of V2R genes from glutamate receptor-family G protein-coupled receptors, most likely the CASR, occurred after the agnathan-gnathostome divergence.
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影响因子：
14.9
作者：
Christoffels, A;van Gelder, A;Hide, W
通讯作者：
Hide, W
影响因子：
56.9
作者：
JONES, DT;REED, RR
通讯作者：
REED, RR
DOI：
10.1073/pnas.0307882100
发表时间：
2004-02-24
影响因子：
11.1
作者：
Malnic, B;Godfrey, PA;Buck, LB
通讯作者：
Buck, LB
影响因子：
2.5
作者：
Laframboise, A. J.;Ren, X.;Zielinski, B. S.
通讯作者：
Zielinski, B. S.
影响因子：
3.8
作者：
Li, W;Sorensen, P W;Gallaher, D D
通讯作者：
Gallaher, D D