Magnetic-field-induced DNA strand breaks in brain cells of the rat.
Magnetic-field-induced DNA strand breaks in brain cells of the rat.
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DOI：
10.1289/ehp.6355
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发表时间：
2004-05
影响因子：
10.4
通讯作者：
Singh NP
中科院分区：
文献类型：
--
作者：
Lai H;Singh NP
在先前的研究中，我们发现大鼠急性（2小时）暴露于强度为0.1-0.5毫特斯拉（mT）的60 Hz正弦磁场中，其脑细胞中的DNA单链和双链断裂增加。进一步的研究表明，这些作用可以通过用自由基清除剂褪黑激素和N-叔丁基-α-苯基硝酮预处理大鼠来阻断，这表明自由基的参与。在本研究中，磁场暴露对大鼠脑细胞DNA的影响进行了进一步研究。暴露于0.01 mT的60 Hz磁场中24小时，导致DNA单链和双链断裂显著增加。将暴露时间延长至48小时导致更大的增加。这表明影响是累积的。此外，用Trolox（维生素E类似物）或7-硝基吲唑（一氧化氮合酶抑制剂）治疗阻断了磁场诱导的DNA链断裂。这些数据进一步支持了自由基对磁场影响的作用。用铁螯合剂去铁酮治疗也阻止了磁场对脑细胞DNA的影响，这表明铁的参与。急性磁场暴露可引起大鼠脑细胞凋亡和坏死。我们假设暴露于60 Hz磁场启动了铁介导的过程（例如，芬顿反应），增加脑细胞中自由基的形成，导致DNA链断裂和细胞死亡。这一假设可能对公共和职业环境中与暴露于极低频磁场相关的可能健康影响具有重要意义。
In previous research, we found that rats acutely (2 hr) exposed to a 60-Hz sinusoidal magnetic field at intensities of 0.1-0.5 millitesla (mT) showed increases in DNA single- and double-strand breaks in their brain cells. Further research showed that these effects could be blocked by pretreating the rats with the free radical scavengers melatonin and N-tert-butyl-alpha-phenylnitrone, suggesting the involvement of free radicals. In the present study, effects of magnetic field exposure on brain cell DNA in the rat were further investigated. Exposure to a 60-Hz magnetic field at 0.01 mT for 24 hr caused a significant increase in DNA single- and double-strand breaks. Prolonging the exposure to 48 hr caused a larger increase. This indicates that the effect is cumulative. In addition, treatment with Trolox (a vitamin E analog) or 7-nitroindazole (a nitric oxide synthase inhibitor) blocked magnetic-field-induced DNA strand breaks. These data further support a role of free radicals on the effects of magnetic fields. Treatment with the iron chelator deferiprone also blocked the effects of magnetic fields on brain cell DNA, suggesting the involvement of iron. Acute magnetic field exposure increased apoptosis and necrosis of brain cells in the rat. We hypothesize that exposure to a 60-Hz magnetic field initiates an iron-mediated process (e.g., the Fenton reaction) that increases free radical formation in brain cells, leading to DNA strand breaks and cell death. This hypothesis could have an important implication for the possible health effects associated with exposure to extremely low-frequency magnetic fields in the public and occupational environments.
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DOI：
10.1016/0006-291x(81)90748-8
发表时间：
1981-01-01
影响因子：
3.1
作者：
FLOYD, RA
通讯作者：
FLOYD, RA
影响因子：
4.5
作者：
Fredenburg, AM;Sethi, RK;Yokel, RA
通讯作者：
Yokel, RA
影响因子：
3.8
作者：
da Silva, RL;Albano, F;Felzenszwalb, I
通讯作者：
Felzenszwalb, I
影响因子：
--
作者：
GRUNDLER, W;KAISER, F;WALLECZEK, J
通讯作者：
WALLECZEK, J
DOI：
10.1007/s00420-003-0446-5
发表时间：
2003-07-01
影响因子：
3
作者：
Ivancsits, S;Diem, E;Rüdiger, HW
通讯作者：
Rüdiger, HW