Fusobacterium nucleatum Infection Induces Malignant Proliferation of Esophageal Squamous Cell Carcinoma Cell by Putrescine Production.
Fusobacterium nucleatum Infection Induces Malignant Proliferation of Esophageal Squamous Cell Carcinoma Cell by Putrescine Production.
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DOI：
10.1128/spectrum.02759-22
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发表时间：
2023-02-22
影响因子：
3.7
通讯作者：
Cui, Yongping
中科院分区：
文献类型：
--
作者：
Ding, Ning;Cheng, Yikun;Liu, Huijuan;Wu, Yueguang;Weng, Yongjia;Cui, Heyang;Cheng, Chen;Zhang, Weimin;Cui, Yongping
食管鳞状细胞癌（ESCC）是一种上消化道恶性肿瘤，其发病机制和病因尚不清楚。由于肠道微生物通常会影响结直肠癌（CRC）的进展、转移和免疫治疗反应，因此食管微生物群在结直肠癌中的作用逐渐引起人们的关注。既往报道，在临床研究中，核梭杆菌（Fusobacterium nucleatum, Fn）作为结直肠癌的“罪魁祸首”，也能影响ESCC的预后。然而，到目前为止，潜在的机制尚不清楚。本研究共收集了73份中国ESCC样本。在这些临床样本中，发现肿瘤中Fn的丰度高于邻近正常组织，并且Fn的高丰度与较短的生存期相关。此外，通过体外实验，我们发现Fn可以侵入ESCC细胞，增强其增殖能力。机制研究表明，Fn侵袭后可产生高水平腐胺，扰乱多胺代谢，促进ESCC细胞恶性增殖。结论：在中国ESCC肿瘤组织样本中发现Fn感染，并可能通过干扰多胺代谢途径促进ESCC的进展。微生物与人体之间复杂多样的相互作用对维持人体健康至关重要。然而，关于食管微生物对食管鳞状细胞癌进展的影响的知识是有限的。在我们的研究中，我们证实了具核梭菌可以侵入ESCC细胞，从而促进其增殖，这表明食道微生物可能在临床环境中影响ESCC的进展。由于食道连接口腔和胃，是运输食物的通道，其特殊的物理位置使其容易接触微生物。因此，有必要探讨食管微生物与ESCC的相互作用。
Esophageal squamous cell carcinoma (ESCC) is a malignant upper digestive tract cancer, and its pathogenesis and etiology are poorly understood. Because gut microbes commonly impact progression, metastasis, and immunotherapy responses in colorectal cancer (CRC), the roles of the esophageal microbiota in ESCC have gradually drawn attention. As reported previously, Fusobacterium nucleatum (Fn), the notable “culprit” of CRC, can also influence the prognosis of ESCC in clinical studies. However, thus far, the underlying mechanism is unclear. In this study, 73 Chinese ESCC samples were collected. In those clinical samples, the abundance of Fn was found to be higher in tumors than in adjacent normal tissues, and a high abundance of Fn was correlated with shorter survival. Furthermore, using in vitro experiments, we demonstrated that Fn can invade ESCC cells, enhancing their proliferation capacity. The mechanism study revealed that Fn can produce high levels of putrescine after invasion, which disturbs polyamine metabolism and promotes the malignant proliferation of ESCC cells. In conclusion, Fn infection was found in Chinese ESCC tumor tissue samples and may promote ESCC progression by disturbing the polyamine metabolism pathway. IMPORTANCE Nowadays, the complex and varied interactions between microbes and human body are known to be crucial for maintaining the health of the human body. However, knowledge concerning the influence of esophageal microbes on the progression of esophageal squamous cell carcinoma is limited. Here, in our study, we confirmed that F. nucleatum can invade ESCC cells and consequently promote their proliferation, suggesting that esophageal microbes likely influence the progression of ESCC in clinical settings. Because the esophagus connects the oral cavity and stomach, acting as a canal for transporting foods, its special physical location makes it easily exposed to microorganisms. Thus, it is necessary to explore the interaction between esophageal microbes and ESCC.
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影响因子：
5.1
作者：
Chen, Wan-qing;Zeng, Hong-mei;He, Jie
通讯作者：
He, Jie
DOI：
10.1186/s13046-020-01677-w
发表时间：
2020-09-29
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Guo P;Tian Z;Kong X;Yang L;Shan X;Dong B;Ding X;Jing X;Jiang C;Jiang N;Yu Y
通讯作者：
Yu Y
影响因子：
8.8
作者：
Liu Y;Baba Y;Ishimoto T;Tsutsuki H;Zhang T;Nomoto D;Okadome K;Yamamura K;Harada K;Eto K;Hiyoshi Y;Iwatsuki M;Nagai Y;Iwagami S;Miyamoto Y;Yoshida N;Komohara Y;Ohmuraya M;Wang X;Ajani JA;Sawa T;Baba H
通讯作者：
Baba H
影响因子：
7
作者：
Castellarin, Mauro;Warren, Rene L.;Holt, Robert A.
通讯作者：
Holt, Robert A.
DOI：
10.1038/s41568-018-0050-3
发表时间：
2018-11
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
Casero RA Jr;Murray Stewart T;Pegg AE
通讯作者：
Pegg AE