Atrial tissue contains a metallo dipeptidyl carboxyhydrolase not present in ventricular tissue: partial purification and characterization.
Atrial tissue contains a metallo dipeptidyl carboxyhydrolase not present in ventricular tissue: partial purification and characterization.
复制标题
心房组织含有心室组织中不存在的金属二肽基羧基水解酶：部分纯化和表征。
DOI：
10.1016/0003-9861(84)90493-4
复制
发表时间：
1984
影响因子：
3.9
通讯作者：
Wilson,IB
中科院分区：
文献类型：
--
作者：
Harris,RB;Wilson,IB
在牛心房组织中鉴定出一种新的膜结合二肽基羧基水解酶，并在用 Triton X-100 提取后已部分纯化。用 Bz-Gly-His-Leu 检测发现，这种酶的含量为 0.01–0.03 单位/克组织，可能能够将心肽 II 水解为心肽 I。该酶位于微粒体部分和富含心房颗粒的蔗糖密度部分中。该酶被 EDTA、邻菲咯啉、二硫苏糖醇和巯基乙醇等重金属试剂完全抑制。后两种化合物也是二硫化物试剂。心房酶也被 d-2-甲基-3-巯基丙酰-l-Pro(卡托普利)、3-巯基丙酰-l-Pro、2-d-甲基琥珀酰-l-Pro 和缓激肽增强因子（所有血管紧张素 I 转换酶的抑制剂）抑制。然而，心房酶在许多动力学和分子特性上不同于转换酶。其活性随着离子强度的增加而增加，但心房酶的 Bz-Gly-His-Leu 水解不具有氯化物依赖性；最适 pH 值 7.3 稍低，并且对非常有效的转化酶抑制剂 d-Cys-l-Pro 的敏感性降低 5500 倍。心房酶的行程半径为 5.00 nm（相比之下为 4.10 nm），其分子量为 240,000（与 145,000 相比）。心室组织不含心房肽，也不含有二肽基羧化水解酶。
A new membrane-bound dipeptidyl carboxyhydrolase has been identified in bovine atrial tissue, and has been partially purified after extraction with Triton X-100. This enzyme, found in quantities of 0.01–0.03 units/g tissue assayed with Bz-Gly-His-Leu, is potentially capable of hydrolyzing atriopeptin II to atriopeptin I. The enzyme is located in the microsomal fraction and in sucrose density fractions enriched for atrial granules. The enzyme is completely inhibited by reagents for heavy metals such as EDTA,o-phenanthroline, dithiothreitol and mercaptoethanol. The latter two compounds are also disulfide reagents. The atrial enzyme is also inhibited byd-2-methyl-3-mercaptopropanoyl-l-Pro(Captopril), 3-mercaptopropanoyl-l-Pro, 2-d-methylsuccinyl-l-Pro, and bradykinin potentiating factor, all inhibitors of the angiotensin I-converting enzyme. However, the atrial enzyme differs from the converting enzyme in a number of kinetic and molecular properties. Its activity increases with ionic strength, but the atrial enzyme does not have a chloride dependence for Bz-Gly-His-Leu hydrolysis; the pH optimum, 7.3, is slightly lower and it is 5500 times less sensitive to the very potent converting enzyme inhibitor,d-Cys-l-Pro. The strokes radius of the atrial enzyme is 5.00 nm as compared to 4.10 nm and its molecular weight is 240,000 compared to 145,000. Ventricular tissue, which does not contain the atrial peptides, does not contain the dipeptidyl carboxyhydrolase enzyme.
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人血浆转化酶。
影响因子：
3.9
作者：
H. Lee;J. N. Larue;I. Wilson
通讯作者：
I. Wilson
大鼠心脏的心房特异性颗粒：光镜染色和组织化学反应。
DOI：
10.1177/26.12.83339
发表时间：
1978
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
J. A.;Bold;J. J.;Raymond
通讯作者：
Raymond
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影响因子：
2.9
作者：
J. Lanzillo;B. Fanburg
通讯作者：
B. Fanburg
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影响因子：
3.9
作者：
Harris,RB;Ohlsson,JT;Wilson,IB
通讯作者：
Wilson,IB
兔睾丸二肽基羧肽酶的分子和催化特性。
影响因子：
4.8
作者：
Hamza A. El;Herbert G. Bull;Kazushi Iwatal;N. Thornberry;E H Cordes;R. L. Soffers
通讯作者：
R. L. Soffers