Statistical Evaluation of Total Expiratory Breath Samples Collected throughout a Year: Reproducibility and Applicability toward Olfactory Sensor-Based Breath Diagnostics.
Statistical Evaluation of Total Expiratory Breath Samples Collected throughout a Year: Reproducibility and Applicability toward Olfactory Sensor-Based Breath Diagnostics.
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DOI：
10.3390/s21144742
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发表时间：
2021-07-11
期刊：
影响因子：
--
通讯作者：
Yoshikawa G
中科院分区：
文献类型：
--
作者：
Inada K;Kojima H;Cho-Isoda Y;Tamura R;Imamura G;Minami K;Nemoto T;Yoshikawa G
呼气中的内源性挥发性有机化合物(VOCs)可作为包括癌症在内的多种疾病的生物标志物。嗅觉传感器有可能从具有特定健康状况的相关VOCs的集体变化中提取特定特征。对于这种方法，重要的是在实际条件下建立一个可行的呼气采样方案，以提供可重现的信号特征。在这里，我们报告了一种稳健的呼吸分析方法，重点是通过膜式表面应力传感器(MSS)测量的总呼气呼吸，该传感器具有人工嗅觉系统的实用特性。为了评估其重复性，在一年多的时间里，从一个受试者身上收集了83个呼气样本。经统计分析证实，通过控制净化室内空气湿度或归一化信号强度来减小湿度对传感信号的影响，可获得合理的重复性。我们还通过区分不同受试者在不同场合饮酒前和饮酒后收集的呼气，证明了该协议在检测目标材料方面的适用性。这一基于MSS嗅觉传感器测量的总呼气呼吸的简单但可重复的方案将有助于探索呼吸诊断临床应用的可能性。
The endogenous volatile organic compounds (VOCs) in exhaled breath can be promising biomarkers for various diseases including cancers. An olfactory sensor has a possibility for extracting a specific feature from collective variations of the related VOCs with a certain health condition. For this approach, it is important to establish a feasible protocol for sampling exhaled breath in practical conditions to provide reproducible signal features. Here we report a robust protocol for the breath analysis, focusing on total expiratory breath measured by a Membrane-type Surface stress Sensor (MSS), which possesses practical characteristics for artificial olfactory systems. To assess its reproducibility, 83 exhaled breath samples were collected from one subject throughout more than a year. It has been confirmed that the reduction of humidity effects on the sensing signals either by controlling the humidity of purging room air or by normalizing the signal intensities leads to reasonable reproducibility verified by statistical analyses. We have also demonstrated the applicability of the protocol for detecting a target material by discriminating exhaled breaths collected from different subjects with pre- and post-alcohol ingestion on different occasions. This simple yet reproducible protocol based on the total expiratory breath measured by the MSS olfactory sensors will contribute to exploring the possibilities of clinical applications of breath diagnostics.
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影响因子：
3.8
作者：
Amann A;Mochalski P;Ruzsanyi V;Broza YY;Haick H
通讯作者：
Haick H
DOI：
10.3390/s16071149
发表时间：
2016-07-22
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Lang HP;Loizeau F;Hiou-Feige A;Rivals JP;Romero P;Akiyama T;Gerber C;Meyer E
通讯作者：
Meyer E
影响因子：
4.6
作者：
Shiba K;Tamura R;Imamura G;Yoshikawa G
通讯作者：
Yoshikawa G
DOI：
10.1007/s11306-017-1241-8
发表时间：
2017
期刊：
Metabolomics : Official journal of the Metabolomic Society
影响因子：
--
作者：
Lawal O;Ahmed WM;Nijsen TME;Goodacre R;Fowler SJ
通讯作者：
Fowler SJ
影响因子：
9
作者：
Kumar, Sacheen;Huang, Juzheng;Hanna, George B.
通讯作者：
Hanna, George B.