The mosquito adulticidal Chromobacterium sp. Panama causes transgenerational impacts on fitness parameters and elicits xenobiotic gene responses.
The mosquito adulticidal Chromobacterium sp. Panama causes transgenerational impacts on fitness parameters and elicits xenobiotic gene responses.
复制标题
DOI：
10.1186/s13071-018-2822-8
复制
发表时间：
2018-04-05
影响因子：
3.2
通讯作者：
Dimopoulos G
中科院分区：
文献类型：
--
作者：
Short SM;van Tol S;Smith B;Dong Y;Dimopoulos G
病媒控制对于减少蚊子造成的疾病负担至关重要，而杀虫剂是控制病媒种群的有效工具。对普通杀虫剂的抗药性现在很普遍，需要新型杀虫剂。在以前的工作中，我们描述了色杆菌属巴拿马（C. sp_P），一种在自然种群中与蚊子相关的细菌的杀蚊活性。在目前的工作中，我们进一步探索了暴露于细菌对蚊子健康和蚊子生理的影响。我们发现C. sp_P对广泛的蚊子类群具有杀蚊活性。成虫暴露于C. sp_P后，雌性安氏按蚊（An.冈比亚的寿命缩短，但生育力没有变化。然而，与对照雌性的后代相比，这些雌性的后代具有较高的幼虫死亡率，并且发育较慢。我们还发现，在从生物膜培养基中去除活细胞后，C. sp_P的杀蚊活性得以保留，表明细菌在生长期间将杀蚊化合物分泌到培养基中。暴露于这种无细胞C. sp_P条件培养基引起雌性中肠转录的变化，包括解毒，异生物质反应和应激反应基因，表明对C. sp_P的生理反应与杀虫剂暴露相似。最后，我们发现色杆菌属的多个成员具有杀蚊活性，但这种活性在用C. sp_P处理的蚊子中最高。我们的研究结果表明，C. sp_P产生对蚊子寿命和适应性具有强烈影响的因子，这可能对杀蚊剂的开发感兴趣。更一般地说，它们表明有必要进一步探索与蚊子相关的和环境微生物以获得新的杀虫化合物或生物防治剂。本文的在线版本（10.1186/s13071 - 018 - 2822 - 8）包含补充材料，可供授权用户使用。
Vector control is critical in reducing the disease burden caused by mosquitoes, and insecticides are an effective tool to control vector populations. Resistance to common insecticides is now widespread, and novel classes of insecticides are needed. In previous work, we described the mosquitocidal activity of Chromobacterium sp. Panama (C.sp_P), a bacterium found in association with mosquitoes in natural populations. In the current work, we further explored the effects of exposure to the bacterium on mosquito fitness and mosquito physiology. We found that C.sp_P has mosquitocidal activity against a broad range of mosquito taxa. When exposed to C.sp_P as adults, female An. gambiae suffered reduced longevity, but experienced no change in fecundity. The offspring of these females, however, had higher mortality as larvae and were slower to develop compared to offspring of control females. We also found that the mosquitocidal activity of C.sp_P was retained after removal of live cells from biofilm culture media, suggesting the bacteria secrete mosquitocidal compound(s) into the media during growth. Exposure to this cell-free C.sp_P-conditioned media caused female midgut transcriptional changes comprising detoxification, xenobiotic response, and stress response genes, suggesting the physiological response to C.sp_P is similar to that of insecticide exposure. Finally, we found that multiple members of the Chromobacterium genus had mosquitocidal activity, but this activity was highest in mosquitoes treated with C.sp_P. Our findings suggest that C.sp_P produces factor(s) with strong effects on mosquito longevity and fitness, which may be of interest for mosquitocide development. More generally, they indicate that further exploration of mosquito-associated and environmental microbes for novel insecticidal compounds or biocontrol agents is warranted. The online version of this article (10.1186/s13071-018-2822-8) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.2
作者：
Gimonneau, Geoffrey;Tchioffo, Majoline T.;Morlais, Isabelle
通讯作者：
Morlais, Isabelle
DOI：
10.1590/s0074-02762007000800005
发表时间：
2007-12-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Gusmão, Desiely Silva;Santos, Adão Valmir;Lemos, Francisco José Alves
通讯作者：
Lemos, Francisco José Alves
DOI：
10.4269/ajtmh.2012.11-0455
发表时间：
2012-04-01
影响因子：
3.3
作者：
Darbro, Jonathan M.;Johnson, Petrina H.;Ryan, Peter A.
通讯作者：
Ryan, Peter A.
蚊子寄生着对发育至关重要的细菌群落，但在当地栖息地之间差异很大。
影响因子：
4.9
作者：
Coon KL;Brown MR;Strand MR
通讯作者：
Strand MR
影响因子：
4.5
作者：
Edi CV;Djogbénou L;Jenkins AM;Regna K;Muskavitch MA;Poupardin R;Jones CM;Essandoh J;Kétoh GK;Paine MJ;Koudou BG;Donnelly MJ;Ranson H;Weetman D
通讯作者：
Weetman D