Manual GO annotation of predictive protein signatures: the InterPro approach to GO curation.
Manual GO annotation of predictive protein signatures: the InterPro approach to GO curation.
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DOI：
10.1093/database/bar068
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hunter S
中科院分区：
文献类型：
--
作者：
Burge S;Kelly E;Lonsdale D;Mutowo-Muellenet P;McAnulla C;Mitchell A;Sangrador-Vegas A;Yong SY;Mulder N;Hunter S
Interpro融合的预测性蛋白质特征来自许多知名的合作伙伴数据库中的单一资源。为了帮助解释结果，在基因本体论（GO）的术语中手动注释了Interpro条目。 Interpro2Go映射由这两个资源之间的交叉引用组成，并且是蛋白质的GO注释预测的最大来源。在这里，我们描述了Interpro策展人将GO术语集成到Interpro数据库中的协议。我们讨论将特定的GO术语与可能描述各种蛋白质的条目集成的独特挑战，我们用示例说明了Invempro层次结构如何反映出提高特异性的术语。我们描述了一个修订后的GO映射协议，使我们能够根据各个域的功能将GO术语分配给域，而不是找到域的家庭功能。我们还讨论了如何处理分类学约束以及无法添加任何适当的GO条款的情况。使用GO术语的专家手册注释可以根据序列匹配到预测模型来推断用户为未表征的序列推断功能，过程或亚细胞信息。
数据库URL：http：//www.ebi.ac.uk/interpro。完整的InterPro2Go映射可用：ftp：//ftp.ebi.ac.uk/pub/databases/goa/goa/goa/goa/extern2go/interpro2go
InterPro amalgamates predictive protein signatures from a number of well-known partner databases into a single resource. To aid with interpretation of results, InterPro entries are manually annotated with terms from the Gene Ontology (GO). The InterPro2GO mappings are comprised of the cross-references between these two resources and are the largest source of GO annotation predictions for proteins. Here, we describe the protocol by which InterPro curators integrate GO terms into the InterPro database. We discuss the unique challenges involved in integrating specific GO terms with entries that may describe a diverse set of proteins, and we illustrate, with examples, how InterPro hierarchies reflect GO terms of increasing specificity. We describe a revised protocol for GO mapping that enables us to assign GO terms to domains based on the function of the individual domain, rather than the function of the families in which the domain is found. We also discuss how taxonomic constraints are dealt with and those cases where we are unable to add any appropriate GO terms. Expert manual annotation of InterPro entries with GO terms enables users to infer function, process or subcellular information for uncharacterized sequences based on sequence matches to predictive models. Database URL: http://www.ebi.ac.uk/interpro. The complete InterPro2GO mappings are available at: ftp://ftp.ebi.ac.uk/pub/databases/GO/goa/external2go/interpro2go
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影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
4.3
作者：
Reference Genome Group of the Gene Ontology Consortium
通讯作者：
Reference Genome Group of the Gene Ontology Consortium
影响因子：
--
作者：
Jensen K;Østergaard PR;Wilting R;Lassen SF
通讯作者：
Lassen SF
影响因子：
14.9
作者：
Lima T;Auchincloss AH;Coudert E;Keller G;Michoud K;Rivoire C;Bulliard V;de Castro E;Lachaize C;Baratin D;Phan I;Bougueleret L;Bairoch A
通讯作者：
Bairoch A
影响因子：
3
作者：
Deegan (nee Clark), Jennifer I.;Dimmer, Emily C.;Mungall, Christopher J.
通讯作者：
Mungall, Christopher J.