Effect of Photoperiod on Chinese Kale (Brassica alboglabra) Sprouts Under White or Combined Red and Blue Light.
Effect of Photoperiod on Chinese Kale (Brassica alboglabra) Sprouts Under White or Combined Red and Blue Light.
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白光或红蓝光组合光周期对羽衣甘蓝芽苗的影响
DOI：
10.3389/fpls.2020.589746
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发表时间：
2020
影响因子：
5.6
通讯作者：
Guo R
中科院分区：
文献类型：
--
作者：
Chen J;Chen Z;Li Z;Zhao Y;Chen X;Wang-Pruski G;Guo R
为了研究芥蓝（Brassica alboglabra）芽苗对不同光周期的响应，采用4种光周期（0 h光照/24 h黑暗、8 h光照/16 h黑暗、12 h光照/12 h黑暗和16 h光照/8 h黑暗），研究了白色和红蓝组合（RB）光源下芥蓝芽苗的生长和次生代谢产物硫代葡萄糖甙（GS）的积累。我们发现，16小时的光条件下RB光产生的植物最大的干物质。在16小时RB光照条件下生长的芽比在白色光照下生长的芽长。为了研究RB光对植物生长和GS积累的作用，采用不同RB比（0：10，2：8，5：5，8：2和10：0）的RB光源培养芽苗。结果表明，在蓝光（RB，0：10）和红光（RB，10：0）条件下生长的芽苗菜，GS积累存在显著差异，蓝光条件下GS含量较高。利用RNA测序技术研究了红光和蓝光条件下芽苗菜GS含量差异的内在机制。有趣的是，丰富的GS生物合成基因的转录本观察到在红光下生长的芽相比，在蓝光下。与GS降解相关的β-葡萄糖苷酶家族同源基因在红光和蓝光条件下的表达存在差异，其中TGG 4同源基因在红光条件下的表达量高于蓝光条件。考虑到在红光下芽苗GS积累较低，我们得出结论，GS的降解可能在芽苗GS稳态中起关键作用。
To determine the response of Chinese kale (Brassica alboglabra) sprouts to photoperiods under different light sources, we used four photoperiods (0-h light/24-h dark, 8-h light/16-h dark, 12-h light/12-h dark, and 16-h light/8-h dark) to investigate their sprout growth and secondary metabolite glucosinolates (GSs) accumulation under white or combined red-and-blue (RB) light sources. We found that the 16-h light condition under RB light produced plants with the greatest dry matter. Sprouts grown under 16-h RB light condition achieved greater length than those under white light. To investigate the role of RB light in plant growth and GS accumulation, we applied RB light sources with different RB ratios (0:10, 2:8, 5:5, 8:2, and 10:0) to cultivate sprouts. The results showed that significant differential accumulation of GSs existed between sprouts grown under blue (RB, 0:10) and red (RB, 10:0) light; there was greater GS content under blue light. The underlying mechanism of differential GS content in sprouts under red or blue light condition was studied using RNA sequencing technique. Interestingly, abundant GS biosynthetic gene transcripts were observed in sprouts grown under red light compared with under blue light. The expression of β-glucosidase family homolog genes related to GS degradation differed under red and blue light conditions, among those TGG4 homolog was detected with higher expression under red light than with blue light. Taking into consideration, the lower GS accumulation in sprouts under red rather than blue light, we conclude that the degradation of GSs may play a key role in sprouts GS homeostasis.
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西兰花（Brassica oleracea var. italic）种子和芽中转录组和芥子油苷代谢的 RNA 测序分析
影响因子：
3.7
作者：
Gao J;Yu X;Ma F;Li J
通讯作者：
Li J
影响因子：
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作者：
Kusnierczyk, Anna;Winge, Per;Bones, Atle Magnar
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DOI：
10.21273/jashs.138.1.31
发表时间：
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影响因子：
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作者：
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作者：
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影响因子：
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