Modification of Serine 1040 of SIBRI1 Increases Fruit Yield by Enhancing Tolerance to Heat Stress in Tomato.
Modification of Serine 1040 of SIBRI1 Increases Fruit Yield by Enhancing Tolerance to Heat Stress in Tomato.
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SIBRI1 丝氨酸 1040 的修饰通过增强番茄对热应激的耐受性来提高果实产量
DOI：
10.3390/ijms21207681
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发表时间：
2020-10-16
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang S;Hu T;Tian A;Luo B;Du C;Zhang S;Huang S;Zhang F;Wang X
高温是影响植物生长和产量的主要环境因子。SlBRI 1是油菜素类固醇信号传导中的关键受体，其磷酸化位点在植物生长和发育中具有不同的功能。然而，SIBRI 1的磷酸化位点在胁迫耐受中的作用尚不清楚。本研究以番茄为材料，研究了SlBRI 1磷酸化位点丝氨酸1040（Ser-1040）的生物学功能。表型分析表明，携带在Ser-1040处去磷酸化的SlBRI 1的转基因番茄表现出对热胁迫的耐受性增加，在高温下表现出比表达SlBRI 1或在Ser-1040处磷酸化的SlBRI 1的转基因株系更好的植物生长和植物产量。生理生化分析进一步表明，抗氧化活性，细胞膜完整性，植物保护剂的积累，光合作用和热胁迫防御基因的转录水平都提高了在番茄植株中携带的SLBRI 1去磷酸化在Ser-1040，和自磷酸化水平的抑制时，SLBRI 1去磷酸化在Ser-1040。综上所述，我们的研究结果表明，SlBRI 1的磷酸化位点Ser-1040影响耐热性，从而改善高温条件下的植物生长和产量。我们的研究结果还表明，磷酸化位点修饰作为一种保护作物产量免受高温胁迫的方法的承诺。
High temperature is a major environmental factor that adversely affects plant growth and production. SlBRI1 is a critical receptor in brassinosteroid signalling, and its phosphorylation sites have differential functions in plant growth and development. However, the roles of the phosphorylation sites of SIBRI1 in stress tolerance are unknown. In this study, we investigated the biological functions of the phosphorylation site serine 1040 (Ser-1040) of SlBRI1 in tomato. Phenotype analysis indicated that transgenic tomato harbouring SlBRI1 dephosphorylated at Ser-1040 showed increased tolerance to heat stress, exhibiting better plant growth and plant yield under high temperature than transgenic lines expressing SlBRI1 or SlBRI1 phosphorylated at Ser-1040. Biochemical and physiological analyses further showed that antioxidant activity, cell membrane integrity, osmo-protectant accumulation, photosynthesis and transcript levels of heat stress defence genes were all elevated in tomato plants harbouring SlBRI1 dephosphorylated at Ser-1040, and the autophosphorylation level of SlBRI1 was inhibited when SlBRI1 dephosphorylated at Ser-1040. Taken together, our results demonstrate that the phosphorylation site Ser-1040 of SlBRI1 affects heat tolerance, leading to improved plant growth and yield under high-temperature conditions. Our results also indicate the promise of phosphorylation site modification as an approach for protecting crop yields from high-temperature stress.
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影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T
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5.6
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作者：
Liu, Jin-Ge;Qin, Qiu-lin;Yao, Quan-Hong
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64.5
作者：
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影响因子：
5.2
作者：
Liu, Hui;Yu, Chuying;Ye, Zhibiao
通讯作者：
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