Enhancing Brassinosteroid Signaling via Overexpression of Tomato (Solanum lycopersicum) SlBRI1 Improves Major Agronomic Traits.
Enhancing Brassinosteroid Signaling via Overexpression of Tomato (Solanum lycopersicum) SlBRI1 Improves Major Agronomic Traits.
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通过番茄 (Solanum lycopersicum) SlBRI1 过度表达增强油菜素类固醇信号传导可改善主要农艺性状
DOI：
10.3389/fpls.2017.01386
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Nie S;Huang S;Wang S;Cheng D;Liu J;Lv S;Li Q;Wang X
类甾醇（BRs）通过其受体--类甾醇不敏感受体1（BRI 1）感知并启动BR信号传导，在植物生长发育和逆境应答中发挥重要作用。调控作物中的BR信号具有相当大的潜在农业价值。在这项研究中，我们研究了过表达番茄（Solanum lycopersicum）BRI 1基因，SLBRI 1，对主要农艺性状，如种子萌发，营养生长，果实乙烯产生，类胡萝卜素积累，产量和品质属性的影响。SlBRI 1过表达增强了内源BR信号强度，从而增加了种子萌发率、侧根数、下胚轴长度、CO2同化、植物高度和花大小。与野生型相比，转基因植株的果实产量和单株果实数也有所增加，但单个果实的平均重量有所降低。SlBRI 1过表达还促进果实成熟和乙烯产生，并引起果实成熟期间类胡萝卜素、抗坏血酸、可溶性固形物和可溶性糖水平的增加。因此，由SlBRI 1过表达介导的BR信号强度增加与类胡萝卜素积累和果实营养品质呈正相关。我们的研究结果表明，通过在番茄中过表达SlBRI 1来增强BR信号传导具有改善多个主要农艺性状的潜力。
Brassinosteroids (BRs) play important roles in plant growth, development, and stress responses through the receptor, Brassinosteroid-insensitive 1 (BRI1), which perceives BRs and initiates BR signaling. There is considerable potential agricultural value in regulating BR signaling in crops. In this study, we investigated the effects of overexpressing the tomato (Solanum lycopersicum) BRI1 gene, SlBRI1, on major agronomic traits, such as seed germination, vegetative growth, fruit ethylene production, carotenoid accumulation, yield, and quality attributes. SlBRI1 overexpression enhanced the endogenous BR signaling intensity thereby increasing the seed germination rate, lateral root number, hypocotyl length, CO2 assimilation, plant height, and flower size. The transgenic plants also showed an increase in fruit yield and fruit number per plant, although the mean weight of individual fruit was reduced, compared with wild type. SlBRI1 overexpression also promoted fruit ripening and ethylene production, and caused an increase in levels of carotenoids, ascorbic acid, soluble solids, and soluble sugars during fruit ripening. An increased BR signaling intensity mediated by SlBRI1 overexpression was therefore positively correlated with carotenoid accumulation and fruit nutritional quality. Our results indicate that enhancing BR signaling by overexpression of SlBRI1 in tomato has the potential to improve multiple major agronomic traits.
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影响因子：
4.3
作者：
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