Sustained and localized in vitro release of BMP-2/7, RANKL, and tetracycline from FlexBone, an elastomeric osteoconductive bone substitute.
Sustained and localized in vitro release of BMP-2/7, RANKL, and tetracycline from FlexBone, an elastomeric osteoconductive bone substitute.
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DOI：
10.1002/jor.20890
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发表时间：
2009-10
影响因子：
2.8
通讯作者：
Song, Jie
中科院分区：
文献类型：
--
作者：
Xu, Jianwen;Li, Xinning;Lian, Jane B.;Ayers, David C.;Song, Jie
关键词：
This study tests the hypothesis that synthetic composites containing a high percentage of osteoconductive biominerals well-integrated with a hydrophilic polymer matrix can be engineered to provide both the structural and biochemical framework of a viable synthetic bone substitute. FlexBone, an elastic hydrogel-mineral composite exhibiting excellent structural integration was prepared by crosslinking pHEMA hydrogel in the presence of 25wt% nanocrystalline hydroxyapatite and 25wt% tricalcium phosphate. Biologically active factors tetracycline, BMP-2/7 and RANKL that stimulate bone formation and remodeling were encapsulated into FlexBone during polymerization or via post-polymerization adsorption. SEM and DMA analyses showed that the encapsulation of tetracycline (5.0wt%) did not compromise the structural integrity and compressive behavior of FlexBone, which could withstand repetitive megapascal-compressive loadings and be securely press-fitted into critical femoral defects. Dose-dependent, sustained in vitro release of tetracycline from FlexBone was characterized by spectroscopy and bacterial inhibition. A single dose of 40-ng-BMP-2/7 or 10-ng-RANKL pre-encapsulated with 50-mg-FlexBone, released over 1 week, was able to locally induce osteogenic differentiation of myoblast C2C12 cells and osteoclastogenesis of macrophage RAW264.7 cells, respectively. With a bone-like structural composition, useful surgical handling characteristics and tunable biochemical microenvironment, FlexBone provides an exciting opportunity for the treatment of hard-to-heal skeletal defects with minimal systemic side effects.
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影响因子：
4.9
作者：
Song, Jie;Xu, Jianwen;Filion, Tera;Saiz, Eduardo;Tomsia, Antoni P.;Lian, Jane B.;Stein, Gary S.;Ayers, David C.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
4.1
作者：
Abarrategi, Ander;Moreno-Vicente, Carolina;Lopez-Lacomba, Jose Luis
通讯作者：
Lopez-Lacomba, Jose Luis
影响因子：
1.8
作者：
Israel, DI;Nove, J;Wozney, JM
通讯作者：
Wozney, JM
DOI：
10.1007/s11420-005-0111-5
发表时间：
2005-09-01
期刊：
HSS journal : the musculoskeletal journal of Hospital for Special Surgery
影响因子：
--
作者：
Bostrom, Mathias P G;Seigerman, Daniel A
通讯作者：
Seigerman, Daniel A
影响因子：
14
作者：
Le Nihouannen, Damien;Hacking, S. Adam;Barralet, Jake E.
通讯作者：
Barralet, Jake E.