Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation.
Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation.
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DOI：
10.1186/s13568-016-0285-x
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发表时间：
2016-12
期刊：
影响因子：
3.7
通讯作者：
Auesukaree C
中科院分区：
文献类型：
--
作者：
Kitichantaropas Y;Boonchird C;Sugiyama M;Kaneko Y;Harashima S;Auesukaree C
高温酒精发酵有几个好处，包括降低冷却成本，最大限度地减少细菌污染的风险，并能够同时糖化和发酵。为了在高温下实现高效的乙醇发酵，酵母菌不仅要能耐受高温，而且要能耐受发酵过程中存在的其他压力，如乙醇、渗透压和氧化压力。C3253、C3751和C4377三株酿酒酵母菌株已被分离为耐热酵母菌，它们对多重胁迫具有耐受性。在这些菌株中，热休克蛋白基因的持续表达和细胞内海藻糖的积累是对引起蛋白质变性的胁迫的响应。与对照菌株相比，这些多重耐受菌株在暴露于几乎所有测试的压力下，显示出较低的细胞内活性氧水平和有效的细胞壁重塑。为了响应同时模拟发酵胁迫的多重胁迫，细胞壁重塑和氧化还原动态平衡似乎是保护细胞免受损伤所需的主要机制。此外，这些菌株在最适温度和高温下都表现出比对照菌株更好的乙醇生产性能，这表明它们在高温酒精发酵中具有潜在的应用前景。
High-temperature ethanol fermentation has several benefits including a reduction in cooling cost, minimizing risk of bacterial contamination, and enabling simultaneous saccharification and fermentation. To achieve the efficient ethanol fermentation at high temperature, yeast strain that tolerates to not only high temperature but also the other stresses present during fermentation, e.g., ethanol, osmotic, and oxidative stresses, is indispensable. The C3253, C3751, and C4377 Saccharomyces cerevisiae strains, which have been previously isolated as thermotolerant yeasts, were found to be multiple stress-tolerant. In these strains, continuous expression of heat shock protein genes and intracellular trehalose accumulation were induced in response to stresses causing protein denaturation. Compared to the control strains, these multiple stress-tolerant strains displayed low intracellular reactive oxygen species levels and effective cell wall remodeling upon exposures to almost all stresses tested. In response to simultaneous multi-stress mimicking fermentation stress, cell wall remodeling and redox homeostasis seem to be the primary mechanisms required for protection against cell damage. Moreover, these strains showed better performances of ethanol production than the control strains at both optimal and high temperatures, suggesting their potential use in high-temperature ethanol fermentation.
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影响因子：
5.4
作者：
Benjaphokee, Suthee;Koedrith, Preeyaporn;Harashima, Satoshi
通讯作者：
Harashima, Satoshi
影响因子：
2.4
作者：
Aguilar-Uscanga, B;François, JM
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François, JM
影响因子：
3.3
作者：
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通讯作者：
Levin, David E.
DOI：
10.1023/a:1001761008817
发表时间：
1998-05-01
影响因子：
2.6
作者：
Kurtzman, CP;Robnett, CJ
通讯作者：
Robnett, CJ
影响因子：
3.8
作者：
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通讯作者：
Yoon, Ho-Sung