Reduced DNA Repair Capacity in Prostate Cancer Patients: A Phenotypic Approach Using the CometChip.
Reduced DNA Repair Capacity in Prostate Cancer Patients: A Phenotypic Approach Using the CometChip.
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前列腺癌患者的DNA修复能力降低：使用cometchip的表型方法。
DOI：
10.3390/cancers14133117
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发表时间：
2022-06-25
期刊：
影响因子：
5.2
通讯作者：
Matta, Jaime
中科院分区：
文献类型：
--
作者：
Ortiz-Sanchez, Carmen;Encarnacion-Medina, Jarline;Park, Jong Y.;Moreno, Natasha;Ruiz-Deya, Gilberto;Matta, Jaime
前列腺癌（PCa）是美国西班牙裔男性中最常见的癌症类型。在西班牙裔中，波多黎各（PR）男性的PCA特异性死亡率最高。各种研究表明，低DNA修复能力（DRC）是癌症发展的重要风险因素。本研究的目的是通过核苷酸切除修复（NER）途径，使用彗星芯片评估PR男性PCa患者DRC的变化。总体而言，PCa病例的DRC低于对照组。当PCa病例分为侵袭性和惰性时，对照组的DRC高于两组。其他因素的贡献（即，年龄和前列腺特异性抗原水平）对DRC的影响。我们的数据表明，DRC水平可能有潜力区分侵略性和惰性的情况下。我们的研究结果代表了基于DRC水平的PCa血液筛查测试的创新一步。前列腺癌（PCa）占美国西班牙裔男性新诊断病例的22%。在西班牙裔中，波多黎各（PR）男性的PCA特异性死亡率最高。使用淋巴细胞功能测定的流行病学研究已经证明，具有低DRC是癌症发展的重要风险因素。本研究的目的是评估PR男性PCa患者DRC的变化。从在医院环境中招募的PCa病例（n = 41）和对照（n = 14）的血液样品中分离淋巴细胞。通过核苷酸切除修复（NER）途径的DRC水平用CometChip使用UVC作为NER诱导剂来测量。对照组和PCa组的平均DRC分别为20.66%（±7.96）和8.41%（±4.88）（p < 0.001）。并探讨DRC与肿瘤侵袭性的关系。进行了额外的比较，以评估年龄，人体测量和前列腺特异性抗原水平的DRC的贡献。这是第一项将CometChip应用于临床癌症研究的研究。我们的研究结果代表了基于DRC水平的PCa血液筛查测试的创新一步。我们的数据还表明，DRC水平可能有潜力区分侵略性和惰性的情况下。
Prostate cancer (PCa) is the most commonly diagnosed cancer type in Hispanic men in the US. Among Hispanics, Puerto Rican (PR) men show the highest PCa-specific mortality. Various studies have shown that having low DNA repair capacity (DRC) is a significant risk factor for cancer development. The aim of this study was to evaluate variations in DRC, through the nucleotide excision repair (NER) pathway, in PR men with PCa using the CometChip. Overall, PCa cases had lower DRC than controls. When PCa cases were stratified into aggressive and indolent, controls had higher DRC than both groups. The contributions of additional factors (i.e., age and prostate-specific antigen levels) to DRC were also considered. Our data suggest that DRC levels may have the potential to discriminate between aggressive and indolent cases. Our results represent an innovative step in the development of a blood-based screening test for PCa based on DRC levels. Prostate cancer (PCa) accounts for 22% of the new cases diagnosed in Hispanic men in the US. Among Hispanics, Puerto Rican (PR) men show the highest PCa-specific mortality. Epidemiological studies using functional assays in lymphocytes have demonstrated that having low DRC is a significant risk factor for cancer development. The aim of this study was to evaluate variations in DRC in PR men with PCa. Lymphocytes were isolated from blood samples from PCa cases (n = 41) and controls (n = 14) recruited at a hospital setting. DRC levels through the nucleotide excision repair (NER) pathway were measured with the CometChip using UVC as a NER inductor. The mean DRC for controls and PCa cases were 20.66% (±7.96) and 8.41 (±4.88), respectively (p < 0.001). The relationship between DRC and tumor aggressiveness was also evaluated. Additional comparisons were performed to evaluate the contributions of age, anthropometric measurements, and prostate-specific antigen levels to the DRC. This is the first study to apply the CometChip in a clinical cancer study. Our results represent an innovative step in the development of a blood-based screening test for PCa based on DRC levels. Our data also suggest that DRC levels may have the potential to discriminate between aggressive and indolent cases.
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影响因子：
3.8
作者：
Matta J;Echenique M;Negron E;Morales L;Vargas W;Gaetan FS;Lizardi ER;Torres A;Rosado JO;Bolaños G;Cruz JG;Laboy J;Barnes R;Medina SS;Romero A;Martinez R;Dutil J;Suarez E;Alvarez-Garriga C;Bayona M
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影响因子：
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作者：
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通讯作者：
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64.5
作者：
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通讯作者：
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影响因子：
2.8
作者：
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影响因子：
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作者：
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通讯作者：
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