Pre-absorbed immunoproteomics: a novel method for the detection of Streptococcus suis surface proteins.
Pre-absorbed immunoproteomics: a novel method for the detection of Streptococcus suis surface proteins.
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预吸收免疫蛋白质组学：检测猪链球菌表面蛋白的新方法
DOI：
10.1371/journal.pone.0021234
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lu C
中科院分区：
文献类型：
--
作者：
Zhang W;Liu G;Tang F;Shao J;Lu Y;Bao Y;Yao H;Lu C
猪链球菌血清2型（SS2）是一种人畜共患病原体，可引起猪和人感染。细菌表面蛋白经常作为潜在的候选疫苗和毒力的生物标志物进行研究。本研究提出了一种结合免疫蛋白质组学和免疫血清学技术鉴定细菌表面蛋白的新方法。这种新方法成功的关键是改进了生成猪链球菌蛋白二维电泳凝胶谱的程序。本研究鉴定的猪链球菌表面蛋白包括muramidase-released protein precursor （MRP）和ABC转运蛋白，而MRP被认为是位于细菌表面的SS2的主要毒力因子之一。在此，我们证明ABC转运蛋白可以结合HEp-2细胞，这强烈表明该蛋白位于细菌细胞表面并可能参与发病机制。免疫荧光测定证实ABC转运蛋白定位于细菌外表面。这种新方法可能被证明是鉴定表面蛋白的有用工具，并有助于开发新的疫苗亚基和疾病诊断。
Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause infections in pigs and humans. Bacterial surface proteins are often investigated as potential vaccine candidates and biomarkers of virulence. In this study, a novel method for identifying bacterial surface proteins is presented, which combines immunoproteomic and immunoserologic techniques. Critical to the success of this new method is an improved procedure for generating two-dimensional electrophoresis gel profiles of S. suis proteins. The S. suis surface proteins identified in this study include muramidase-released protein precursor (MRP) and an ABC transporter protein, while MRP is thought to be one of the main virulence factors in SS2 located on the bacterial surface. Herein, we demonstrate that the ABC transporter protein can bind to HEp-2 cells, which strongly suggests that this protein is located on the bacterial cell surface and may be involved in pathogenesis. An immunofluorescence assay confirmed that the ABC transporter is localized to the bacterial outer surface. This new method may prove to be a useful tool for identifying surface proteins, and aid in the development of new vaccine subunits and disease diagnostics.
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影响因子：
3.3
作者：
Gottschalk, M;Segura, M
通讯作者：
Segura, M
影响因子：
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