Comparative genomics reveals cotton-specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium.
Comparative genomics reveals cotton-specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium.
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比较基因组学揭示了大丽黄萎病灵活基因组区域中棉花特异性毒力因子以及镰刀菌水平基因转移的证据
DOI：
10.1111/nph.14861
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Dai XF
中科院分区：
文献类型：
--
作者：
Chen JY;Liu C;Gui YJ;Si KW;Zhang DD;Wang J;Short DPG;Huang JQ;Li NY;Liang Y;Zhang WQ;Yang L;Ma XF;Li TG;Zhou L;Wang BL;Bao YM;Subbarao KV;Zhang GY;Dai XF
大丽花黄萎病分离株对最初分离它们的宿主是最毒的。这些显性宿主适应的机制目前尚不清楚。我们对大丽花V. dahliae Vd991的基因组进行了测序，并与JR2（来自番茄）和vdl的参考基因组进行了比较(从生菜)。进行了致病性相关因素预测、同源学和多基因家族分类、转录组分析、系统发育分析和致病性实验。Vd991基因组在LS区域（LSRs）中包含了几个排他性的、谱系特异性的（LS）基因。Vd991中一个LSR （G‐LSR2）内的7个基因缺失突变体仅对棉花的毒力较弱。G‐LSR2基因单独整合到JR2和vdl中。17对棉花毒力显著增强，对番茄和生菜毒力无明显影响。与其他寄主相比，Vd991在棉花侵染早期的7个LS基因转录水平较高。系统发育分析表明，G‐LSR2是通过水平基因转移从vasinfection Fusarium oxysporum f. sp.获得的。我们的研究结果证明，镰刀菌向Vd991的水平基因转移显著促进了其对棉花的适应，并可能代表了一种无性植物病原体进化的重要机制。
Verticillium dahliae isolates are most virulent on the host from which they were originally isolated. Mechanisms underlying these dominant host adaptations are currently unknown. We sequenced the genome of V. dahliae Vd991, which is highly virulent on its original host, cotton, and performed comparisons with the reference genomes of JR2 (from tomato) and VdLs.17 (from lettuce). Pathogenicity‐related factor prediction, orthology and multigene family classification, transcriptome analyses, phylogenetic analyses, and pathogenicity experiments were performed. The Vd991 genome harbored several exclusive, lineage‐specific (LS) genes within LS regions (LSRs). Deletion mutants of the seven genes within one LSR (G‐LSR2) in Vd991 were less virulent only on cotton. Integration of G‐LSR2 genes individually into JR2 and VdLs.17 resulted in significantly enhanced virulence on cotton but did not affect virulence on tomato or lettuce. Transcription levels of the seven LS genes in Vd991 were higher during the early stages of cotton infection, as compared with other hosts. Phylogenetic analyses suggested that G‐LSR2 was acquired from Fusarium oxysporum f. sp. vasinfectum through horizontal gene transfer. Our results provide evidence that horizontal gene transfer from Fusarium to Vd991 contributed significantly to its adaptation to cotton and may represent a significant mechanism in the evolution of an asexual plant pathogen.
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影响因子：
30.8
作者：
Friesen, Timothy L.;Stukenbrock, Eva H.;Oliver, Richard P.
通讯作者：
Oliver, Richard P.
影响因子：
7
作者：
Birney, E;Durbin, R
通讯作者：
Durbin, R
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
影响因子：
4.5
作者：
Coleman JJ;Rounsley SD;Rodriguez-Carres M;Kuo A;Wasmann CC;Grimwood J;Schmutz J;Taga M;White GJ;Zhou S;Schwartz DC;Freitag M;Ma LJ;Danchin EG;Henrissat B;Coutinho PM;Nelson DR;Straney D;Napoli CA;Barker BM;Gribskov M;Rep M;Kroken S;Molnár I;Rensing C;Kennell JC;Zamora J;Farman ML;Selker EU;Salamov A;Shapiro H;Pangilinan J;Lindquist E;Lamers C;Grigoriev IV;Geiser DM;Covert SF;Temporini E;Vanetten HD
通讯作者：
Vanetten HD
DOI：
10.1073/pnas.1424293112
发表时间：
2015-03-17
影响因子：
11.1
作者：
Dhillon, Braham;Feau, Nicolas;Hamelin, Richard C.
通讯作者：
Hamelin, Richard C.