Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration.
Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration.
复制标题
在核迁移方面，需要与酿酒酵母的动力学相关的KIP3。
DOI：
10.1083/jcb.138.5.1023
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发表时间：
1997-09-08
影响因子：
7.8
通讯作者：
Roof, DM
中科院分区：
文献类型：
--
作者：
DeZwaan, TM;Ellingson, E;Pellman, D;Roof, DM
纺锤体定向和核迁移是许多真核生物细胞生长和分化的关键事件。在这里，我们表明，KIP 3，第六和最后的驱动蛋白相关基因在酿酒酵母，是必需的细胞核迁移到芽的网站，准备有丝分裂。通过固定细胞的显微镜和单个活细胞的延时显微镜检查细胞核在细胞中的位置和有丝分裂纺锤体的方向。KIP3和动力蛋白重链基因的突变定义了核迁移的两个不同阶段：KIP3依赖的细胞核向早期芽位的移动和后期细胞核通过芽颈的动力蛋白依赖的易位。KIP3功能的丧失破坏了细胞核朝向芽和有丝分裂纺锤体方向的单向运动，导致细胞核位置的大振荡。振荡运动有时使细胞核靠近芽颈，可能是kip3无效突变体的生存能力。kip3无效突变体表现出正常易位的核通过颈部和正常的纺锤体极分离动力学在后期。KIP 3和驱动蛋白相关的KAR 3功能的同时丧失，或KIP 3和动力蛋白功能的同时丧失，是致命的，但不阻断任何额外的可检测到的运动。这表明致死性是由于连续和可能重叠的缺陷的组合。表位标记的Kip3p定位于星形和中央纺锤体微管，也存在于整个细胞质和细胞核。
Spindle orientation and nuclear migration are crucial events in cell growth and differentiation of many eukaryotes. Here we show that KIP3, the sixth and final kinesin-related gene in Saccharomyces cerevisiae, is required for migration of the nucleus to the bud site in preparation for mitosis. The position of the nucleus in the cell and the orientation of the mitotic spindle was examined by microscopy of fixed cells and by time-lapse microscopy of individual live cells. Mutations in KIP3 and in the dynein heavy chain gene defined two distinct phases of nuclear migration: a KIP3-dependent movement of the nucleus toward the incipient bud site and a dynein-dependent translocation of the nucleus through the bud neck during anaphase. Loss of KIP3 function disrupts the unidirectional movement of the nucleus toward the bud and mitotic spindle orientation, causing large oscillations in nuclear position. The oscillatory motions sometimes brought the nucleus in close proximity to the bud neck, possibly accounting for the viability of a kip3 null mutant. The kip3 null mutant exhibits normal translocation of the nucleus through the neck and normal spindle pole separation kinetics during anaphase. Simultaneous loss of KIP3 and kinesin-related KAR3 function, or of KIP3 and dynein function, is lethal but does not block any additional detectable movement. This suggests that the lethality is due to the combination of sequential and possibly overlapping defects. Epitope-tagged Kip3p localizes to astral and central spindle microtubules and is also present throughout the cytoplasm and nucleus.
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DOI：
10.1083/jcb.138.3.629
发表时间：
1997-08-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carminati JL;Stearns T
通讯作者：
Stearns T
DOI：
10.1083/jcb.105.5.2123
发表时间：
1987-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hyman AA;White JG
通讯作者：
White JG
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
通讯作者：
Saunders WS
影响因子：
7.8
作者：
FARKASOVSKY, M;KUNTZEL, H
通讯作者：
KUNTZEL, H
影响因子：
64.5
作者：
MELUH, PB;ROSE, MD
通讯作者：
ROSE, MD