Expression of glutathione S-transferase alpha, P1-1 and T1-1 in the human gastrointestinal tract.
Expression of glutathione S-transferase alpha, P1-1 and T1-1 in the human gastrointestinal tract.
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DOI：
10.1111/j.1349-7006.2000.tb00946.x
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发表时间：
2000-03
期刊：
影响因子：
--
通讯作者：
Peters WH
中科院分区：
文献类型：
--
作者：
de Bruin WC;Wagenmans MJ;Peters WH
谷胱甘肽S -转移酶（GSTs）形成一个酶家族，它通过解毒许多潜在的致癌化合物在预防癌症中发挥重要作用。gst催化谷胱甘肽与这些有害分子结合，并使其分泌。人类的消费税可分为五大类。θ类同工酶最近才被鉴定出来，而且这些酶的免疫组织化学数据有限。本研究采用免疫组织化学方法对不同胃肠道组织石蜡包埋切片进行GSTα、GSTP1-1和GSTT1-1特异性抗体的表达分析。GSTα、GSTP1-1和GSTT1-1在所有胃肠道组织中均高表达，具有独特的细胞分布。GSTT1-1是第一个在十二指肠Paneth细胞和腺中发现的GST同工酶。GSTα、GSTT1-1和GSTP1-1在人体胃肠道的许多细胞类型中普遍表达，表明其在抵抗致癌物和其他外源物方面具有重要作用。
Glutathione S‐transferases (GSTs) form a family of enzymes, which play an important role in the prevention of cancer by detoxifying numerous potentially carcinogenic compounds. GSTs catalyze the conjugation of glutathione to such harmful molecules, and enable their secretion. Human GSTs can be divided into five main classes. The θ class of isoenzymes was only recently identified and limited (immunohistochemical) data on these enzymes are available. In the present study, paraffinembedded sections of different gastrointestinal tissues were analyzed immunohistochemically for GSTα, GSTP1–1 and GSTT1–1 expression using specific antibodies. GSTα, GSTP1–1 and GSTT1–1 were highly expressed in all gastrointestinal tissues examined, with a unique cellular distribution. GSTT1–1 is the first GST isoenzyme demonstrated in duodenal Paneth cells and glands of Brunner. The common expression of GSTα, GSTT1–1 and GSTP1–1 in many cell types along the human gastrointestinal tract suggests an important role in the protection against carcinogens and other xenobiotics.
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影响因子：
4.1
作者：
Sherratt, PJ;Pulford, DJ;Hayes, JD
通讯作者：
Hayes, JD
影响因子：
4.1
作者：
HUSSEY, AJ;HAYES, JD
通讯作者：
HAYES, JD
影响因子：
4.7
作者：
MULDER, TPJ;ROELOFS, HMJ;VERSPAGET, HW
通讯作者：
VERSPAGET, HW
影响因子：
4.1
作者：
Mainwaring, GW;Williams, SM;Green, T
通讯作者：
Green, T
影响因子：
8.8
作者：
PETERS, WHM;ROELOFS, HMJ;JANSEN, JBMJ
通讯作者：
JANSEN, JBMJ