Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem.
Three-dimensional macronutrient-associated Fos expression patterns in the mouse brainstem.
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DOI：
10.1371/journal.pone.0008974
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发表时间：
2010-02-01
期刊：
影响因子：
3.7
通讯作者：
Darcel N
中科院分区：
文献类型：
--
作者：
Schwarz J;Burguet J;Rampin O;Fromentin G;Andrey P;Tomé D;Maurin Y;Darcel N
尾侧脑干在短期饱足和控制进食终止方面发挥着重要作用。由胃肠道（GI）感知的与进食相关的刺激通过血流传递到最后区（AP），或通过迷走神经传递到孤束核（NTS）。关于尾侧脑干中大量营养素特异性信号的编码知之甚少。我们假设蔗糖和酪蛋白胨激活NTS神经元的空间上不同的亚群，从而使用统计三维建模表征后者。使用免疫标记的原癌基因Fos作为神经元活动的标记，结合统计三维建模方法，我们已经表明，NTS神经元激活的蔗糖或蛋白胨灌胃占据不同的，虽然部分重叠，位置。具体而言，当比较蛋白胨处理的小鼠中的同源物，从蔗糖灌胃后的神经元密度图计算的三维模型显示，Fos阳性神经元占据更多的侧位在NTS的吻端，和更多的背侧位置在尾端。据我们所知，这是第一次，NTS神经元的亚群已区分根据其功能反应的空间组织。这种神经元活动模式可能与理解支持消化期间胃肠道中大量营养素存在相关的信号的中枢编码的机制特别相关。最后，这一发现也说明了有用的统计三维建模功能神经解剖学研究。
The caudal brainstem plays an important role in short-term satiation and in the control of meal termination. Meal-related stimuli sensed by the gastrointestinal (GI) tract are transmitted to the area postrema (AP) via the bloodstream, or to the nucleus tractus solitarii (NTS) via the vagus nerve. Little is known about the encoding of macronutrient-specific signals in the caudal brainstem. We hypothesized that sucrose and casein peptone activate spatially distinct sub-populations of NTS neurons and thus characterized the latter using statistical three-dimensional modeling. Using immunolabeling of the proto-oncogene Fos as a marker of neuronal activity, in combination with a statistical three-dimensional modeling approach, we have shown that NTS neurons activated by sucrose or peptone gavage occupy distinct, although partially overlapping, positions. Specifically, when compared to their homologues in peptone-treated mice, three-dimensional models calculated from neuronal density maps following sucrose gavage showed that Fos-positive neurons occupy a more lateral position at the rostral end of the NTS, and a more dorsal position at the caudal end. To our knowledge, this is the first time that subpopulations of NTS neurons have be distinguished according to the spatial organization of their functional response. Such neuronal activity patterns may be of particular relevance to understanding the mechanisms that support the central encoding of signals related to the presence of macronutrients in the GI tract during digestion. Finally, this finding also illustrates the usefulness of statistical three-dimensional modeling to functional neuroanatomical studies.
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影响因子：
5.8
作者：
Bowen, J;Noakes, M;Clifton, PM
通讯作者：
Clifton, PM
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
DOI：
10.1002/ar.a.20088
发表时间：
2004-09-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Berthoud, HR
通讯作者：
Berthoud, HR
DOI：
10.1152/ajpgi.1999.277.4.g751
发表时间：
1999-10-01
影响因子：
4.5
作者：
Raybould, HE
通讯作者：
Raybould, HE
影响因子：
2.5
作者：
NORGREN, R;SMITH, GP
通讯作者：
SMITH, GP