Optimization of the Ultrasonic-Assisted Extraction Technology of Steroidal Saponins from Polygonatum kingianum Collett & Hemsl and Evaluating Its Quality Planted in Different Areas.
Optimization of the Ultrasonic-Assisted Extraction Technology of Steroidal Saponins from Polygonatum kingianum Collett & Hemsl and Evaluating Its Quality Planted in Different Areas.
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不同产地黄精中甾体皂苷的超声辅助提取工艺优化及品质评价
DOI：
10.3390/molecules27051463
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发表时间：
2022-02-22
期刊：
影响因子：
--
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
He S;Wang X;Chen J;Li X;Gu W;Zhang F;Cao G;Yu J
滇黄精（Polygonatum kingianum Collett & Hemsl）是具有益气养阴、润肺等功效的传统中药之一，甾体皂苷是其重要的活性成分之一。目的：确定姜半夏甾体皂苷的最佳提取工艺，并对5个不同产地姜半夏进行质量评价。通过单因素试验和响应面法优化超声波辅助提取薯蓣皂苷的工艺条件，建立了薯蓣皂苷和薯蓣皂苷元的高效液相色谱测定方法。结果表明，超声波辅助提取法是一种有效的甾体皂苷提取方法，液料比对提取率有显著影响。结果表明，最佳提取工艺条件为：料液比10：1（mL/g）、乙醇浓度85%（v/v）、提取时间75 min、提取温度50 °C、提取3次，各参数均与响应面法预测值相符。仅以薯蓣皂苷和薯蓣皂苷元计，5个样地种植的滇贝母质量差异不显著。而屏边地区薯蓣皂苷元含量最高，为0.46mg/g。通过响应面法确定了姜皮木甾体皂苷的最佳UAE工艺。质量评价结果表明，不同产地的滇贝母中薯蓣皂苷和薯蓣皂苷元的含量差异不显著。该研究对姜木的开发利用具有重要的参考价值。
Polygonatum kingianum Collett & Hemsl is one of the famous traditional Chinese herbs with satisfactory therapeutic effects on invigorating Qi, nourishing Yin and moistening lungs, in which steroidal saponins are one class of important active substances. The main purpose is to determine the optimal extraction technology of steroidal saponins and evaluate the quality of P. kingianum planted in five different areas. The optimal ultrasonic-assisted extraction (UAE) technology was established by using single-factor experiments and the response surface methodology (RSM), and the determination method of high-performance liquid chromatography (HPLC) for dioscin and diosgenin, two primary types of acid-hydrolyzed steroidal saponins, was constructed with good linear range and precision. The results showed that UAE was an efficient extraction method for steroidal saponins, and the extraction yield was significantly affected by the liquid-solid ratio. The optimal extraction technology was generated following a liquid-solid ratio of 10:1 (mL/g), an ethanol concentration of 85% (v/v), an extraction time of 75 min, an extraction temperature of 50 °C and three extractions, of which these parameters were in line with the predicted values calculated by RSM. Considering only dioscin and diosgenin, the quality of P. kingianum planted at five sample plots presented non-significant difference. However, the content of diosgenin in Pingbian Prefecture (PB) was higher than that of the other four areas with a value of 0.46 mg/g. Taken together, the optimal UAE technology for P. kingianum steroidal saponins was determined via RSM. The quality evaluation revealed that there was a non-significant difference among P. kingianum planted in different areas based on the contents of the sum of dioscin and diosgenin. This work has important reference value for the exploitation and utilization of P. kingianum.
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