Identification of cell types in human diseased corneas.
Identification of cell types in human diseased corneas.
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人类患病角膜细胞类型的鉴定。
DOI：
10.1097/00003226-200104000-00014
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发表时间：
2001
期刊：
影响因子：
2.8
通讯作者：
Brown,DJ
中科院分区：
文献类型：
--
作者：
Kenney,MC;Chwa,M;Lin,B;Huang,GH;Ljubimov,AV;Brown,DJ
目的：在角膜创伤模型中经常发现活化的肌成纤维细胞和巨噬细胞。方法收集正常人、圆锥角膜、圆锥角膜积水、大泡性角膜病变、MAP斑点指纹营养不良、植片失败、酸烧伤/新生血管形成的角膜标本，OCT冰冻切片，免疫组织化学染色。结果：正常和MAP-Dot-指纹营养不良的角膜缺乏抗体和酶染色。圆锥角膜CD68、人类白细胞抗原DR和溶酶体酶阳性，CD14和平滑肌肌动蛋白阴性。大泡性角膜病变的角膜有CD68、CD14和人类白细胞抗原DR阳性细胞，相对正常的酶水平，而平滑肌肌动蛋白阴性。移植失败的角膜有大量CD68、CD14和人类白细胞抗原DR阳性细胞，酸性磷酸酶升高，但这些角膜是平滑肌肌动蛋白阴性的。溃烂和血管化的角膜对所有检查的抗体均呈阳性染色。培养的正常角膜基质细胞CD68阳性，CD14阴性，α-平滑肌肌动蛋白阴性，它们产生溶酶体酶。结论：目前的研究表明，在没有肌成纤维细胞和/或巨噬细胞的情况下，溶酶体酶的存在增加，角膜重塑和纤维化发生。
Purpose.Activated myofibroblasts and macrophages are often found in corneal wound models. The current study was performed to determine whether human diseased corneas that had active tissue remodeling and enzyme activities also possessed myofibroblasts, macrophages, major histocompatibility complex class II cells, and/or CD-68–positive cells.Methods.Normal, keratoconus, keratoconus with hydrops, bullous keratopathy, map-dot-fingerprint dystrophy, failed grafts, and acid burn/neovascularized corneas were collected, frozen in OCT, sectioned, and stained with antibodies to alpha smooth muscle actin (myofibroblast marker), CD14 (macrophage marker), CD68 (lysosomal membrane marker), and HLA-DR (major histocompatibility complex class II cells). Selective histochemical stains identified lysosomal enzymes.Results.Normal and map-dot-fingerprint dystrophy corneas lacked antibody and enzyme staining. Keratoconus corneas were positive for CD68, HLA-DR, and lysosomal enzymes but were negative for CD14 and smooth muscle actin. Bullous keratopathy corneas had CD68-, CD14-, and HLA-DR–positive cells, relatively normal enzyme levels, and were smooth muscle actin-negative. Failed graft corneas had significant numbers of CD68-, CD14-, and HLA-DR–positive cells and increased acid phosphatase, but these corneas were smooth muscle actin-negative. Ulcerated and vascularized corneas had positive staining with all antibodies that were examined. Cultured stromal cells from normal corneas were CD68-positive, CD14-negative, and alpha smooth muscle actin-negative, and they produced lysosomal enzymes.Conclusions.The current study demonstrates that increased presence of lysosomal enzymes, corneal remodeling, and fibrosis can occur in the absence of myofibroblasts and/or macrophages.
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DOI：
--
发表时间：
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期刊：
British Journal of Preventive and Social Medicine
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DOI：
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发表时间：
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期刊：
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DOI：
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发表时间：
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期刊：
影响因子：
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作者：
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DOI：
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发表时间：
1983
期刊：
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作者：
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影响因子：
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作者：
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通讯作者：
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