Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.
Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.
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基于碳点的荧光抗体纳米探针用于脑肿瘤胶质母细胞瘤诊断
DOI：
10.1039/d2na00060a
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发表时间：
2022-03-29
影响因子：
4.7
通讯作者：
Galan MC
中科院分区：
文献类型：
--
作者：
Ghirardello M;Shyam R;Liu X;Garcia-Millan T;Sittel I;Ramos-Soriano J;Kurian KM;Galan MC
开发有效和灵敏的工具来检测患者的脑癌是极其重要的，特别是因为这些肿瘤中的许多在疾病进展和治疗效果较差时才被诊断出来。目前的策略使用抗体(Abs)来检测组织样本中的胶质纤维酸性蛋白(GFAP)，因为GFAP是大脑独有的，不存在于正常的外周血液中，而且它依赖于荧光记者。在这里，我们描述了一种低成本、实用和通用的方法，用于用荧光碳点(CD)标记蛋白质和抗体，以产生坚固、光稳定和适用于临床的诊断探针。两步法依赖于二苯并环辛炔(DBCO)官能化的CD与叠氮官能化蛋白质的偶联，通过结合酰胺偶联和应变促进的炔-叠氮环加成(SPAAC)连接化学。用这种策略开发的新型抗体-CD偶联物被成功地用于人脑胶质母细胞瘤(GBM)患者脑组织的免疫组织化学染色，验证了该方法的有效性。总体而言，这些新型荧光探针在成本、光稳定性和适用性方面提供了一种有前景的和通用的策略，可以扩展到其他抗体和蛋白质系统。碳点标记抗体用于人脑组织免疫荧光染色。
The development of efficient and sensitive tools for the detection of brain cancer in patients is of the utmost importance particularly because many of these tumours go undiagnosed until the disease has advanced and when treatment is less effective. Current strategies employ antibodies (Abs) to detect Glial Fibrillary Acid Protein (GFAP) in tissue samples, since GFAP is unique to the brain and not present in normal peripheral blood, and it relies on fluorescent reporters. Herein we describe a low cost, practical and general method for the labelling of proteins and antibodies with fluorescent carbon dots (CD) to generate diagnostic probes that are robust, photostable and applicable to the clinical setting. The two-step protocol relies on the conjugation of a dibenzocyclooctyne (DBCO)-functionalised CD with azide functionalised proteins by combining amide conjugation and strain promoted alkyne–azide cycloaddition (SPAAC) ligation chemistry. The new class of Ab-CD conjugates developed using this strategy was successfully used for the immunohistochemical staining of human brain tissues of patients with glioblastoma (GBM) validating the approach. Overall, these novel fluorescent probes offer a promising and versatile strategy in terms of costs, photostability and applicability which can be extended to other Abs and protein systems. Carbon dot-labelled antibody for human brain tissue immunofluorescent staining.
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DOI：
10.3390/nano11081877
发表时间：
2021-07-22
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Ghirardello M;Ramos-Soriano J;Galan MC
通讯作者：
Galan MC
影响因子：
4.7
作者：
通讯作者：
--
影响因子：
8.4
作者：
Akkapeddi P;Azizi SA;Freedy AM;Cal PMSD;Gois PMP;Bernardes GJL
通讯作者：
Bernardes GJL
影响因子：
2.7
作者：
Hill S;Galan MC
通讯作者：
Galan MC
基于水溶性发光碳纳米点的生物相容性荧光墨水
影响因子：
16.6
作者：
Qu, Songnan;Wang, Xiaoyun;Wang, Lijun
通讯作者：
Wang, Lijun