Selective photothermal killing of cancer cells using LED-activated nucleus targeting fluorescent carbon dots.
Selective photothermal killing of cancer cells using LED-activated nucleus targeting fluorescent carbon dots.
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DOI：
10.1039/c9na00293f
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发表时间：
2019-08-06
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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作者：
由于选择性和脱靶毒性问题，癌症治疗中有效治疗诊断探针的开发受到阻碍。我们报告了选择性的LED光热消融宫颈癌细胞（HeLa）在人类皮肤成纤维细胞（HDF）使用一类新的绿色发射荧光碳点（FCD）。FCD可以使用廉价的商业原料在一锅中容易地制备。物理化学表征显示，在坚固的无定形核上的2，5-脱氧果糖嗪的表面涂层产生了纳米材料的独特性质。我们表明，细胞内摄取主要涉及与细胞内DNA相互作用相结合的被动机制，以靶向细胞核，并且癌细胞选择性杀伤可能是由于细胞内温度升高与ATP耗竭相结合，这在暴露于“裸”核心FCD或单独的表面组分时未观察到。这些纳米探针的选择性和缺乏明显的有毒代谢副产物的产生使这些新的纳米材料在癌症治疗中成为有前途的药物。在癌症治疗中有效的治疗诊断探针仍然是一个挑战。我们报告了选择性杀死癌细胞光热消融后，使用一类新的2，5-脱氧果糖胺涂层的绿色发射碳量子点，使用温和的LED照射。
The development of effective theranostic probes in cancer therapy is hampered due to issues with selectivity and off-target toxicity. We report the selective LED-photothermal ablation of cervical (HeLa) cancer cells over human dermal fibroblasts (HDF) using a new class of green-emissive fluorescent carbon dots (FCDs). The FCDs can be easily prepared in one pot using cheap and commercial starting materials. Physico-chemical characterization revealed that a surface coating of 2,5-deoxyfructosazine on a robust amorphous core gives rise to the nanomaterial's unique properties. We show that intracellular uptake mostly involves passive mechanisms in combination with intracellular DNA interactions to target the nucleus and that cancer cell selective killing is likely due to an increase in intracellular temperature in combination with ATP depletion, which is not observed upon exposure to either the “naked” core FCDs or the surface components individually. The selectivity of these nanoprobes and the lack of apparent production of toxic metabolic byproducts make these new nanomaterials promising agents in cancer therapy. Effective theranostic probes in cancer therapy are still a challenge. We report the selective killing of cancer cells upon photothermal ablation using a new class of 2,5-deoxyfructosazine-coated green-emissive carbon dots using mild LED-irradiation.
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DOI：
10.3402/nano.v1i0.4889
发表时间：
2010
期刊：
Nano reviews
影响因子：
--
作者：
Lévy R;Shaheen U;Cesbron Y;Sée V
通讯作者：
Sée V
影响因子：
13.3
作者：
Bartczak, Dorota;Muskens, Otto L.;Kanaras, Antonios G.
通讯作者：
Kanaras, Antonios G.
影响因子：
6.1
作者：
Bhattacherjee, Abhishek;Hrynets, Yuliya;Betti, Mirko
通讯作者：
Betti, Mirko
影响因子：
16.1
作者：
Janib, Siti M.;Moses, Ara S.;MacKay, J. Andrew
通讯作者：
MacKay, J. Andrew
影响因子：
2.7
作者：
Hill S;Galan MC
通讯作者：
Galan MC