Feeding cues and injected nutrients induce acute expression of multiple clock genes in the mouse liver.
Feeding cues and injected nutrients induce acute expression of multiple clock genes in the mouse liver.
复制标题
DOI：
10.1371/journal.pone.0023709
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kobori M
中科院分区：
文献类型：
--
作者：
Oike H;Nagai K;Fukushima T;Ishida N;Kobori M
生物钟与能量代谢密切相关。肝脏生物钟可以在几天内迅速适应新的喂养周期，而肺生物钟则需要一周时间才能逐渐适应。然而，组织特异性时钟重置的机制尚未完全理解。为了表征肝脏时钟中对进食提示的快速反应，我们检查了单次延时进食对Per 2：：Luc报告基因敲入小鼠肝脏和肺部昼夜节律的影响。在适应夜间限制喂养时间表后，在最后一天根据4、8或13小时延迟时间表喂养小鼠。在延迟喂食的所有组中，肝脏时钟的相位延迟，而肺时钟不受影响。然后，我们使用聚焦DNA微阵列检查了肝脏中时钟和代谢相关基因的急性反应。在恒定光照下饲养时钟突变小鼠，以减弱内源性昼夜节律，并在禁食24小时，然后喂食8小时期间测定基因表达谱。Per 2和Dec 1在1 h内显著增加。实时RT-PCR分析显示，在禁食过夜后喂养野生型小鼠引起的肝时钟基因表达的类似急性反应。除Per 2和Dec 1外，在禁食后进食1 h内，肝脏中Per 1的表达增加，Rev-erbα的表达减少，而这些时钟基因在肺中均未受到影响。此外，腹膜内注射葡萄糖结合氨基酸，但不是单独的，再现了类似的肝脏反应。我们的研究结果表明，多个时钟基因在1小时内对肝脏中的营养提示做出反应，但在肺中没有。
The circadian clock is closely associated with energy metabolism. The liver clock can rapidly adapt to a new feeding cycle within a few days, whereas the lung clock is gradually entrained over one week. However, the mechanism underlying tissue-specific clock resetting is not fully understood. To characterize the rapid response to feeding cues in the liver clock, we examined the effects of a single time-delayed feeding on circadian rhythms in the liver and lungs of Per2::Luc reporter knockin mice. After adapting to a night-time restricted feeding schedule, the mice were fed according to a 4, 8, or 13 h delayed schedule on the last day. The phase of the liver clock was delayed in all groups with delayed feeding, whereas the lung clock remained unaffected. We then examined the acute response of clock and metabolism-related genes in the liver using focused DNA-microarrays. Clock mutant mice were bred under constant light to attenuate the endogenous circadian rhythm, and gene expression profiles were determined during 24 h of fasting followed by 8 h of feeding. Per2 and Dec1 were significantly increased within 1 h of feeding. Real-time RT-PCR analysis revealed a similarly acute response in hepatic clock gene expression caused by feeding wild type mice after an overnight fast. In addition to Per2 and Dec1, the expression of Per1 increased, and that of Rev-erbα decreased in the liver within 1 h of feeding after fasting, whereas none of these clock genes were affected in the lung. Moreover, an intraperitoneal injection of glucose combined with amino acids, but not either alone, reproduced a similar hepatic response. Our findings show that multiple clock genes respond to nutritional cues within 1 h in the liver but not in the lung.
登录
查看更多内容
DOI：
10.1016/j.bbrc.2010.09.072
发表时间：
2010-11-05
影响因子：
3.1
作者：
Oike, Hideaki;Nagai, Kanji;Kobori, Masuko
通讯作者：
Kobori, Masuko
DOI：
10.1016/s0006-291x(02)02427-0
发表时间：
2002-10-25
影响因子：
3.1
作者：
Oishi, K;Miyazaki, K;Ishida, N
通讯作者：
Ishida, N
影响因子：
5.3
作者：
Abe, M;Herzog, ED;Block, GD
通讯作者：
Block, GD
DOI：
10.1152/ajpendo.90617.2008
发表时间：
2008-12-01
影响因子：
5.1
作者：
Andrikopoulos, Sofianos;Blair, Amy R.;Proietto, Joseph
通讯作者：
Proietto, Joseph
影响因子：
2.7
作者：
Nakamura, Takahiro J.;Sellix, Michael T.;Kudo, Takashi;Nakao, Nobuhiro;Yoshimura, Takashi;Ebihara, Shizufumi;Colwell, Christopher S.;Block, Gene D.
通讯作者：
Block, Gene D.