Somatic homologous recombination in plants is promoted by a geminivirus in a tissue-selective manner.

Somatic homologous recombination in plants is promoted by a geminivirus in a tissue-selective manner.
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双生病毒以组织选择性方式促进植物中的体细胞同源重组

DOI:
10.1016/j.virol.2014.01.024
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发表时间:
2014
期刊:
影响因子:
3.7
通讯作者:
Kleinow
Kleinow
中科院分区:
医学3区
文献类型:
--
作者:
Richter;Kleinow

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采用4种携带不同报告基因构建的基于分裂型葡糖苷酸酶基因的转基因拟南芥拟碱类植物,监测双病毒感染后体细胞同源重组的频率。之所以选择大戟花叶病毒和克莱莫叶皱病毒作为例子,是因为它们只会引起轻微的症状,而且与其他双病毒相比,预计它们不会引起普遍的应激反应。通过比较不同的植物系和病毒,并优化侵染程序,大胡花叶病毒在转基因报告系1445中显著提高了重组率。这种效应在叶脉细胞中是组织特异性的,正如对这种韧皮部限制型病毒的预期。本文讨论了双病毒激活一般重组途径的优势,并参考了通过产生病毒重组体来增加适应度,这种重组体经常被观察到是一种流行病学驱动力。
Four transgenicArabidopsis thalianalines carrying different reporter gene constructs based on split glucuronidase genes were used to monitor the frequency of somatic homologous recombination after geminivirus infections.Euphorbia mosaic virusandCleome leaf crumple viruswere chosen as examples, because they induce only mild symptoms and are expected to induce less general stress responses than other geminiviruses. After comparing the different plant lines and viruses as well as optimizing the infection procedure,Euphorbia mosaic virusenhanced recombination rates significantly in the transgenic reporter line 1445. The effect was tissue-specific in cells of the leaf veins as expected for this phloem-limited virus. The advantage for geminiviruses to activate a general recombination pathway is discussed with reference to an increased fitness by generating virus recombinants which have been observed frequently as an epidemiologic driving force.
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