Injectable bone cement with magnesium-containing microspheres enhances osteogenesis via anti-inflammatory immunoregulation.
Injectable bone cement with magnesium-containing microspheres enhances osteogenesis via anti-inflammatory immunoregulation.
复制标题
含镁微球可注射骨水泥通过抗炎免疫调节增强成骨作用
DOI：
10.1016/j.bioactmat.2021.03.006
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发表时间：
2021-10
影响因子：
18.9
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Tan S;Wang Y;Du Y;Xiao Y;Zhang S
可注射骨水泥在微创手术中特别有用，可修复小而不规则的骨缺损。在各种可注射骨水泥中，生物活性磷酸钙骨水泥（CPC）因其生物活性而被广泛研究。然而，其致密的结构和差的生物降解性阻止活组织的向内生长，这导致不期望的骨再生和临床转化。为了解决这个问题，我们制备了基于含镁微球（MMSs）的骨水泥，其不仅可以固化成3D多孔支架，而且还具有可控的生物降解性，连续为所需的组织向内生长提供空间。有趣的是，从MMSs骨水泥（MMSC）释放的镁离子通过上调抗炎基因IL-10和M2巨噬细胞极化以及增加的CD 206表达来触发正性免疫调节，这有利于骨生成。此外，MMSC的物理化学性质，包括热释放、流变性和凝固时间，可以调节以满足临床应用的可注射骨水泥的要求。使用大鼠模型，我们已经证明，MMSC促进成骨通过调解组织向内生长和抗炎免疫调节。该研究为设计和制备具有三维多孔结构、生物降解性和抗炎免疫调节作用的可注射骨水泥提供了范例，以有效促进成骨。采用改进的液滴凝聚法成功制备了含镁微球。MMSs骨水泥具有三维多孔结构，具有生物降解性和Mg 2+介导的抗炎骨免疫调节作用。为设计和制备具有所需成骨作用的新型可注射骨水泥提供了一种新的策略。
Injectable bone cement is especially useful in minimally invasive surgeries to repair small and irregular bone defects. Amongst different kinds of injectable bone cements, bioactive calcium phosphate bone cement (CPC) has been widely studied due to its biological activity. However, its dense structure and poor biodegradability prevent the ingrowth of living tissue, which leads to undesirable bone regeneration and clinical translation. To address this issue, we prepared bone cement based on Magnesium-containing microspheres (MMSs) that can not only be cured into a 3D porous scaffold but also have controllable biodegradability that continuously provides space for desired tissue ingrowth. Interestingly, magnesium ions released from MMSs cement (MMSC) trigger positive immunomodulation via upregulation of the anti-inflammatory genes IL-10 and M2 macrophage polarization with increased expression of CD206, which is beneficial to osteogenesis. Moreover, the physicochemical properties of MMSC, including heat release, rheology and setting time, can be tuned to meet the requirements of injectable bone cement for clinical application. Using a rat model, we have demonstrated that MMSC promoted osteogenesis via mediation of tissue ingrowth and anti-inflammatory immunomodulation. The study provides a paradigm for the design and preparation of injectable bone cements with 3D porous structures, biodegradability and anti-inflammatory immunoregulation to efficiently promote osteogenesis. Magnesium containing microspheres (MMSs) were prepared successfully by a modified liquid drop condensation method. MMSs cement had 3D porous structure with biodegradability and anti-inflammatory osteoimmunomodulation mediated by the Mg2+. A new strategy was provided for the design and preparation of novel injectable bone cements with desired osteogenesis.
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DOI：
10.1016/0267-6605(94)90018-3
发表时间：
1994-01-01
期刊：
Clinical Materials
影响因子：
--
作者：
Ginebra, M. P.;Fernandez, E.;Driessens, F. C. M.
通讯作者：
Driessens, F. C. M.
影响因子：
14
作者：
Sadowska, Joanna M.;Wei, Fei;Xiao, Yin
通讯作者：
Xiao, Yin
影响因子：
24.2
作者：
Chen, Zetao;Klein, Travis;Xiao, Yin
通讯作者：
Xiao, Yin
影响因子：
14
作者：
Du Y;Liu H;Yang Q;Wang S;Wang J;Ma J;Noh I;Mikos AG;Zhang S
通讯作者：
Zhang S
影响因子：
14
作者：
Liu, Changsheng;Shao, Huifang;Zheng, Haiyan
通讯作者：
Zheng, Haiyan