Incrimination of shrews as a reservoir for Powassan virus.
Incrimination of shrews as a reservoir for Powassan virus.
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DOI：
10.1038/s42003-021-02828-1
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发表时间：
2021-11-22
影响因子：
5.9
通讯作者：
Telford SR 3rd
中科院分区：
文献类型：
--
作者：
Goethert HK;Mather TN;Johnson RW;Telford SR 3rd
鲍瓦桑病毒谱系2(鹿虱病毒)是对美国公共卫生的紧急威胁，导致严重的神经系统疾病。它在自然界中的生命周期仍然知之甚少。我们使用宿主特异的逆转录转座子靶向实时聚合酶链式反应方法来检验这样的假设，即白足小鼠被认为是莱姆病联合感染剂的主要宿主，是鹿虱病毒的宿主。在20只感染病毒的寻找宿主的黑腿若虫中，65%以地鼠为食，无一种以老鼠为食。在一个地点，扁虱以地鼠为食的比例与病毒感染的流行率呈正相关，但与莱姆病病原体无关。在一只地鼠的大脑中检测到病毒RNA。我们的结论是，地鼠可能是鹿虱病毒的储存宿主，宿主血粉分析可以为指控储存宿主提供直接证据，从而促进我们对扁虱传播感染生态的了解。Goethert和他的同事使用宿主特定的反转录转座子靶向实时聚合酶链式反应分析来寻找若虫，以确定鲍瓦桑病毒谱系2(或鹿虱病毒)的储存库。被感染的扁虱被发现优先以地鼠为食，而不是像预期的那样以白脚鼠为食，这表明它们可能是这种病毒的储存宿主。
Powassan virus lineage 2 (deer tick virus) is an emergent threat to American public health, causing severe neurologic disease. Its life cycle in nature remains poorly understood. We use a host-specific retrotransposon-targeted real time PCR assay to test the hypothesis that white-footed mice, considered the main eastern U.S. reservoir of the coinfecting agent of Lyme disease, is the reservoir for deer tick virus. Of 20 virus-infected host-seeking nymphal black-legged ticks 65% fed on shrews and none on mice. The proportion of ticks feeding on shrews at a site is positively associated with prevalence of viral infection, but not the Lyme disease agent. Viral RNA is detected in the brain of one shrew. We conclude that shrews are a likely reservoir host for deer tick virus and that host bloodmeal analysis can provide direct evidence to incriminate reservoir hosts, thereby promoting our understanding of the ecology of tick-borne infections. Goethert and colleagues use a host-specific retrotransposon targeted real time PCR assay on questing nymphal ticks to identify a reservoir for Powassan virus lineage 2 (or deer tick virus). Infected ticks were found to have preferentially fed on shrews, as opposed to white-footed mice as was expected, suggesting that they may be a reservoir host for this virus.
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影响因子：
2.9
作者：
Hartemink, N. A.;Randolph, S. E.;Heesterbeek, J. A. P.
通讯作者：
Heesterbeek, J. A. P.
DOI：
10.4269/ajtmh.2000.63.36
发表时间：
2000-07-01
影响因子：
3.3
作者：
Ebel, GD;Campbell, EN;Telford, SR
通讯作者：
Telford, SR
影响因子：
3.8
作者：
Ebel, GD;Spielman, A;Telford, SR
通讯作者：
Telford, SR
影响因子：
11.8
作者：
El Khoury, Marc Y.;Hull, Rene C.;Wormser, Gary P.
通讯作者：
Wormser, Gary P.
影响因子：
4.8
作者：
Tokarz, Rafal;Tagliafierro, Teresa;Lipkin, W. Ian
通讯作者：
Lipkin, W. Ian