Automated phenotyping of plant shoots using imaging methods for analysis of plant stress responses - a review.

Automated phenotyping of plant shoots using imaging methods for analysis of plant stress responses - a review.
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DOI:
10.1186/s13007-015-0072-8
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发表时间:
2015
期刊:
影响因子:
5.1
通讯作者:
Spíchal L
Spíchal L
中科院分区:
生物学2区
文献类型:
--
作者:
Humplík JF;Lazár D;Husičková A;Spíchal L

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目前的内部植物表型分析方法为植物生物学研究提供了强大的新工具。在过去几年中建立的自建和商业平台,在大规模和高通量规模上采用非破坏性方法和测量。这些平台在一定程度上提供了自动化测量,使用简单的单传感器分析或多传感器的高级综合同时分析。然而,由于所使用的方法的复杂性,并不总是清楚这些形式的植物表型可以为潜在的最终用户,即植物生物学家提供什么。本文综述了植物芽表型分析中的成像方法,包括所用传感器的简要调查。为了向更广泛的受众开放这一主题,我们在这里简单介绍了自动无损分析的原理,即RGB,叶绿素荧光,热和高光谱成像。我们进一步概述了如何以及在何种程度上,自动化综合内部表型分析平台最近已被用于研究植物对各种变化环境的反应。
Current methods of in-house plant phenotyping are providing a powerful new tool for plant biology studies. The self-constructed and commercial platforms established in the last few years, employ non-destructive methods and measurements on a large and high-throughput scale. The platforms offer to certain extent, automated measurements, using either simple single sensor analysis, or advanced integrative simultaneous analysis by multiple sensors. However, due to the complexity of the approaches used, it is not always clear what such forms of plant phenotyping can offer the potential end-user, i.e. plant biologist. This review focuses on imaging methods used in the phenotyping of plant shoots including a brief survey of the sensors used. To open up this topic to a broader audience, we provide here a simple introduction to the principles of automated non-destructive analysis, namely RGB, chlorophyll fluorescence, thermal and hyperspectral imaging. We further on present an overview on how and to which extent, the automated integrative in-house phenotyping platforms have been used recently to study the responses of plants to various changing environments.
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