Interact to survive: Phyllobacterium brassicacearum improves Arabidopsis tolerance to severe water deficit and growth recovery.
Interact to survive: Phyllobacterium brassicacearum improves Arabidopsis tolerance to severe water deficit and growth recovery.
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DOI：
10.1371/journal.pone.0107607
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Vile D
中科院分区：
文献类型：
--
作者：
Bresson J;Vasseur F;Dauzat M;Labadie M;Varoquaux F;Touraine B;Vile D
共生细菌可以改变植物的表型，赋予植物新的能力。一些植物促生根际细菌（PGPR）是已知的，以提高植物的生长和多种胁迫的耐受性，包括干旱，但在严重缺水的植物生存的影响的报告很少。我们研究了从油菜根际分离的PGPR--芸苔叶杆菌STM 196菌株对严重水分亏缺下拟南芥的存活、生长和生理反应的影响，并结合破坏性和非破坏性高通量表型分析。土壤接种STM 196可显著提高A.在几种严重缺水的情况下，光系统II的效率，在整个植物水平的高通量荧光成像（Fv/Fm），评估有关的生存概率，并透露，STM 196延迟植物死亡率。接种存活植株通过延迟脱水和更好的耐低水分状态，耐受更多的损害光合组织。重要的是，STM 196允许在再浇水后更好地恢复植物生长，并且胁迫植物在开花时达到与非胁迫植物相似的生物量。我们的研究结果强调了植物-细菌相互作用在植物对严重干旱的反应中的重要性，并为提高农业耐旱性提供了一条新的研究途径。
Mutualistic bacteria can alter plant phenotypes and confer new abilities to plants. Some plant growth-promoting rhizobacteria (PGPR) are known to improve both plant growth and tolerance to multiple stresses, including drought, but reports on their effects on plant survival under severe water deficits are scarce. We investigated the effect of Phyllobacterium brassicacearum STM196 strain, a PGPR isolated from the rhizosphere of oilseed rape, on survival, growth and physiological responses of Arabidopsis thaliana to severe water deficits combining destructive and non-destructive high-throughput phenotyping. Soil inoculation with STM196 greatly increased the survival rate of A. thaliana under several scenarios of severe water deficit. Photosystem II efficiency, assessed at the whole-plant level by high-throughput fluorescence imaging (F v/F m), was related to the probability of survival and revealed that STM196 delayed plant mortality. Inoculated surviving plants tolerated more damages to the photosynthetic tissues through a delayed dehydration and a better tolerance to low water status. Importantly, STM196 allowed a better recovery of plant growth after rewatering and stressed plants reached a similar biomass at flowering than non-stressed plants. Our results highlight the importance of plant-bacteria interactions in plant responses to severe drought and provide a new avenue of investigations to improve drought tolerance in agriculture.
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影响因子：
6.4
作者：
BENGTSON, C;FALK, SO;LARSSON, S
通讯作者：
LARSSON, S
影响因子：
6.4
作者：
Galmes, Jeroni;Abadia, Anunciacion;Flexas, Jaume
通讯作者：
Flexas, Jaume
影响因子：
6.5
作者：
Bertrand, H;Nalin, R;Cleyet-Marel, JC
通讯作者：
Cleyet-Marel, JC
影响因子：
6.4
作者：
Gray, GR;Hope, BJ;Whitehead, CL
通讯作者：
Whitehead, CL
影响因子：
4.8
作者：
Figueiredo, Marcia V. B.;Burity, Helio A.;Chanway, Christopher P.
通讯作者：
Chanway, Christopher P.