Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia.
Human adipose tissue‑derived stem cells alleviate radiation‑induced xerostomia.
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人类脂肪组织来源的干细胞减轻辐射引起的口干症
DOI：
10.3892/ijmm.2014.1837
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发表时间：
2014-09
影响因子：
5.4
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Xiong X;Shi X;Chen F
唾液分泌减少是头颈部肿瘤放射治疗的一个难治性副作用。它是由腺泡细胞的不可逆损失和唾液分泌减少引起的。然而，这种情况严重损害了受影响患者的生活质量。目前，对这种情况没有有效的治疗方法。在本研究中，我们开发了一种新的方法来再生的辐射损伤的唾液腺使用人脂肪组织来源的干细胞（hADSC）腺内移植的功能。在暴露于18戈伊照射后立即将ZsGreen标记的hADSC过继转移到Sprague-Dawley（SD）大鼠下颌下腺中。在hADSC治疗组中观察到更高的唾液流率（SFR）。与未处理的腺体相比，在hADSC处理的腺体中检测到组织改善，包括血管生成、抗凋亡和抗纤维化。定量逆转录PCR（RT-qPCR）显示hADSC治疗组大鼠血管内皮生长因子（VEGF）、肝细胞生长因子（HGF）、环氧合酶-2（考克斯-2）和基质金属蛋白酶-2（MMP-2）的表达显著增加。免疫组化结果显示，大鼠下颌下腺中hADSCs已分化为腺泡细胞和导管细胞。因此，我们的研究结果表明，hADSCs能够通过腺体移植再生辐射损伤的唾液腺。
Hyposalivation is an intractable side-effect of radiotherapy for head and neck cancer. It is caused by the irreversible loss of acinar cells and decreased saliva secretion. However, this situation severely compromises the quality of life of affected patients. Currently, there is no effective treatment for this condition. In the present study, we developed a novel approach to regenerate the function of the irradiation-damaged salivary glands using human adipose tissue-derived stem cell (hADSC) intraglandular transplantation. ZsGreen-labeled hADSCs were adoptively transferred into Sprague-Dawley (SD) rat submandibular glands immediately following exposure to 18 Gy irradiation. A higher salivary flow rate (SFR) was observed in the hADSC-treated group. Tissue improvement, including angiogenesis, anti-apoptosis and anti-fibrosis, was detected in the hADSC-treated glands as compared to the untreated glands. Quantitative reverse transcription PCR (RT-qPCR) revealed a significantly higher expression of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), cyclooxygenase-2 (COX-2) and matrix metalloproteinase-2 (MMP-2) in the hADSC-treated rats. Furthermore, immunohistochemical analysis indicated that the hADSCs had differentiated into acinar and ductal cells in the rat submandibular glands. Thus, our results suggest that hADSCs are able to regenerate irradiation-damaged salivary glands through glandular transplantation.
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干细胞移植在辐照腺体中的干细胞移植后拯救唾液腺功能。
影响因子：
3.7
作者：
Lombaert, Isabelle M. A.;Brunsting, Jeanette F.;Wierenga, Pieter K.;Faber, Hette;Stokman, Monique A.;Kok, Tineke;Visser, Willy H.;Kampinga, Harm H.;de Haan, Gerald;Coppes, Robert P.
通讯作者：
Coppes, Robert P.
影响因子：
18.6
作者：
Baum, BJ;Tran, SD
通讯作者：
Tran, SD
影响因子：
3.1
作者：
Amerongen, AVN;Veerman, ECI
通讯作者：
Veerman, ECI
影响因子：
6.5
作者：
Erices, A;Conget, P;Minguell, JJ
通讯作者：
Minguell, JJ
影响因子：
3.3
作者：
Zuk, PA;Zhu, M;Hedrick, MH
通讯作者：
Hedrick, MH