Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly.
Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly.
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DOI：
10.1038/s41467-023-37915-w
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发表时间：
2023-05-11
影响因子：
16.6
通讯作者：
Gold, Vicki A. M.
中科院分区：
文献类型：
--
作者：
Conners, Rebecca;Leon-Quezada, Rayen Ignacia;McLaren, Mathew;Bennett, Nicholas J.;Daum, Bertram;Rakonjac, Jasna;Gold, Vicki A. M.
噬菌体是感染细菌的病毒，并主宰着我们星球上的每一个生态系统。除了影响微生物生态学、生理学和进化外，微生物还被用作分子生物学和生物技术的工具。这对于代表广泛分布的丝状病毒组的Ff（f1、fd或M13）病毒尤其如此。在近五十年的时间里，Ffs已经有了广泛的应用，但噬菌体衣壳的完整结构以及感染和组装的机制仍然是个谜。在这项工作中，我们使用冷冻电子显微镜和一个高效的系统，用于生产短FF衍生的纳米棒，以确定包括尖端的丝状病毒的结构。我们表明，结构结合诱变可以识别噬菌体结构域，是重要的细菌攻击和释放新的后代，允许新的模型提出的噬菌体生命周期。在这项工作中，作者报告了一种生产丝状噬菌体短版本的系统，该系统能够通过冷冻电子显微镜确定结构。结合诱变的结构允许鉴定在细菌攻击和释放新病毒后代中重要的噬菌体结构域。
Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ffs have seen an extraordinary range of applications, yet the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. In this work, we use cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods to determine a structure of a filamentous virus including the tips. We show that structure combined with mutagenesis can identify phage domains that are important in bacterial attack and for release of new progeny, allowing new models to be proposed for the phage lifecycle. In this work, the authors report a system for production of short versions of a filamentous phage enables the structure to be determined by cryo-electron microscopy. Structure combined with mutagenesis allows the identification of phage domains that are important in bacterial attack and for release of new viral progeny.
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影响因子：
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