First identification of NDM-4-producing Escherichia coli ST410 in China.
First identification of NDM-4-producing Escherichia coli ST410 in China.
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国内首次鉴定产NDM-4大肠杆菌ST410。
DOI：
10.1038/emi.2016.117
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发表时间：
2016-11-23
影响因子：
13.2
通讯作者：
Feng, Xianju
中科院分区：
文献类型：
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作者：
Qin, Shangshang;Zhou, Mengmeng;Zhang, Qijing;Tao, Hengxun;Ye, Yafei;Chen, Huizhi;Xu, Lijuan;Xu, Hui;Wang, Ping;Feng, Xianju
新德里金属β-内酰胺酶1 （NDM-1）是一种Ambler B类金属β-内酰胺酶（MBL），它对除单巴坦类外的所有β-内酰胺类具有耐药性，在全球范围内传播，引起了公众健康的极大关注。NDM-4与NDM-1只存在一个氨基酸取代（Met154Leu）的差异，具有更高的碳青霉烯酶活性。作为一种罕见的blaNDM等位基因，仅在印度、奥地利和欧洲报道过几例由产生NDM-4的大肠杆菌引起的散发感染病例。目前，只有一个NDM-4质粒（pJEG027； GenBank登录NO KM400601）被鉴定。为了了解耐药病原体的流行病学和控制其传播，我们对中国人类患者中碳青霉烯类耐药肠杆菌科进行了调查。此前，我们报道了ndm -1阳性肠杆菌科在河南省的高发病率（33.3%）和地方性传播。5在这里，我们描述了第一个产生ndm -4的大肠杆菌ST410分离物，据我们所知，这是中国的第一个病例，并报告了携带IncX3质粒的blaNDM-4的特性。大肠杆菌14-55于2014年6月6日从河南省郑州市郑州大学第一附属医院住院的一名14岁女孩的血培养物中获得。这名女孩没有出国旅行的历史。药敏试验最初采用Vitek 2系统（biomrieux, Marcy l’etoile, France）进行，最低抑制浓度（MIC）采用肉汤微量稀释法和琼脂稀释法（用于磷霉素），并根据CLSI断点解释MIC结果。6 .欧洲抗微生物药物敏感性测试委员会的断点（可在http://www上找到）。eucast。/clinical_breakpoints/)用于粘菌素和替加环素。大肠杆菌14-55对所有β-内酰胺类耐药，包括亚胺培南和美罗培南、氟喹诺酮类、庆大霉素和四环素，对阿米卡星、磷霉素、替加环素和粘菌素敏感（补充表S1）。改良的Hodge试验和亚胺培烯- edta双盘协同试验表明，大肠杆菌14-55是一种产生mbl的菌株。6 .采用PCR和测序方法筛选MBL基因，包括blaIMP、blaNDM、blaVIM；只检测到blaNDM。5利用blaNDM基因两侧的一对引物（NDM-F: 5′-GAA TTC GCC CCA TAT TTT TGC; NDM-R: 5′-AAC GCC TCT GTC ACA TCG AAA T）扩增出blaNDM的完整编码序列（CDS）， DNA测序结果显示大肠杆菌14-55中存在blaNDM-4。此外，在NDM-4阳性分离株大肠杆菌14-55中鉴定出不同的β-内酰胺酶编码基因blaCTX-M-15、blaSHV-12、blaTEM-1和blaCMY-61以及qnrB基因。5大肠杆菌系统发育类群分型和多位点序列分型（MLST）表明，分离株大肠杆菌14-55属于A和ST410系统群。5,7目前，在全球范围内已发现5个大肠杆菌STs（印度：ST648；意大利和丹麦：ST405；奥地利：ST167、ST4450和ST101）携带blaNDM-4。1-3因此，本研究首次报道了ST410作为blaNDM-4的载体。值得注意的是，最近来自德国和意大利的出版物描述了产生氟喹诺酮（FQ）耐药ctx - m -15的大肠杆菌ST410在人类和动物群体中的克隆扩增。8,9我们之前和目前的研究还发现大肠杆菌ST410临床分离株共携带多种耐药决定因子，包括blaNDM （blaNDM-1/4）、ESBLS…
Dear Editor, The worldwide dissemination of New Delhi metallo-β-lactamase 1 (NDM-1), an Ambler class B metallo-β-lactamase (MBL) conferring resistance to all β-lactams except monobactams, is of great concern for public health. NDM-4, which differs from NDM-1 by a single amino acid substitution (Met154Leu), was demonstrated to possess increased carbapenemase activity. 1 As an infrequent blaNDM allele, only several sporadic cases of infections due to NDM-4-producing Escherichia coli have been described in India, Austria, and Europe. 1–3 At present, only one NDM-4 plasmid (pJEG027; GenBank accession NO KM400601) has been characterized. 4 To understand the epidemiology and control the spread of antibiotic-resistant pathogens, we have been investigating carbapenem-resistant Enterobacteriaceae from human patients in China. Previously, we reported a high incidence (33.3%) and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province. 5 Here we describe the first NDM-4-producing E. coli ST410 isolate, which, to our knowledge, is the first case in China, and report the characterization of the blaNDM-4 harboring IncX3 plasmid. E. coli 14–55 was obtained from a blood culture of a 14-year-old girl hospitalized at The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, Henan province, on 6 June 2014. The girl did not have a history of foreign travel. Susceptibility testing was initially performed by a Vitek 2 system (bioMérieux, Marcy l'Etoile, France), and Minimum Inhibitory Concentrations (MICs) were determined using the broth microdilution method and the agar dilution method (for fosfomycin) following the CLSI guidelines, and the MIC results were interpreted according to the CLSI breakpoints. 6 The European Committee on Antimicrobial Susceptibility Testing breakpoints (available at http://www. eucast. org/clinical_breakpoints/) were used for colistin and tigecycline. E. coli 14–55 was resistant to all β-lactams, including imipenem and meropenem, fluoroquinolones, gentamicin and tetracycline, and was susceptible to amikacin, fosfomycin, tigecycline and colistin (Supplementary Table S1). The modified Hodge test and the imipenem-EDTA double-disk synergy test revealed that E. coli 14–55 was an MBL-producer. 6 PCR and sequencing were performed to screen for the presence of MBL genes, including blaIMP, blaNDM, and blaVIM; only blaNDM was detected. 5 The complete coding sequence (CDS) of blaNDM was amplified with a pair of primers (NDM-F: 5′-GAA TTC GCC CCA TAT TTT TGC; NDM-R: 5′-AAC GCC TCT GTC ACA TCG AAA T) that flanked the blaNDM gene, and DNA sequencing revealed the presence of blaNDM-4 in E. coli 14–55. In addition, different β-lactamase encoding genes, including blaCTX-M-15, blaSHV-12, blaTEM-1 and blaCMY-61, together with the qnrB gene were identified in the NDM-4 positive isolate E. coli 14–55. 5 E. coli phylogenetic group typing and multilocus sequence typing (MLST) showed that the isolate E. coli 14–55 belonged to phylogroup A and ST410. 5, 7 At present, five E. coli STs (India: ST648; Italy and Denmark: ST405; Austria: ST167, ST4450, and ST101) have been identified carrying blaNDM-4 worldwide. 1–3 Thus, this study represents the first report of ST410 as a carrier of blaNDM-4. Notably, recent publications from Germany and Italy described the clonal expansion of fluoroquinolone (FQ)-resistant CTX-M-15-producing E. coli ST410 in both human and animal populations. 8, 9 Our previous and current studies also identified E. coli ST410 clinical isolates co-harboring multiple resistance determinants, including blaNDM (blaNDM-1/4), ESBLS …
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DOI：
10.1128/genomea.00757-15
发表时间：
2015-07-09
期刊：
Genome announcements
影响因子：
--
作者：
Giufré M;Accogli M;Graziani C;Busani L;Cerquetti M
通讯作者：
Cerquetti M
影响因子：
4.9
作者：
Chen, L.;Peirano, G.;Pitout, J. D.
通讯作者：
Pitout, J. D.
影响因子：
13.2
作者：
Ho, Pak-Leung;Li, Zhen;Lo, Wai-U;Cheung, Yuk-Yam;Lin, Chi-Ho;Sham, Pak-Chung;Cheng, Vincent Chi-Chung;Ng, Tak-Keung;Que, Tak-Lun;Chow, Kin-Hung
通讯作者：
Chow, Kin-Hung
影响因子：
4.9
作者：
Wailan, Alexander M.;Paterson, David L.;Sidjabat, Hanna E.
通讯作者：
Sidjabat, Hanna E.
影响因子：
4.9
作者：
Nordmann, Patrice;Boulanger, Anne E.;Poirel, Laurent
通讯作者：
Poirel, Laurent