Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies.
Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies.
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DOI：
10.1038/s41419-018-0990-2
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发表时间：
2018-09-17
影响因子：
9
通讯作者：
Vescovi AL
中科院分区：
文献类型：
--
作者：
Rosati J;Ferrari D;Altieri F;Tardivo S;Ricciolini C;Fusilli C;Zalfa C;Profico DC;Pinos F;Bernardini L;Torres B;Manni I;Piaggio G;Binda E;Copetti M;Lamorte G;Mazza T;Carella M;Gelati M;Valente EM;Simeone A;Vescovi AL
Establishing specific cell lineages from human induced pluripotent stem cells (hiPSCs) is vital for cell therapy approaches in regenerative medicine, particularly for neurodegenerative disorders. While neural precursors have been induced from hiPSCs, the establishment of hiPSC-derived human neural stem cells (hiNSCs), with characteristics that match foetal hNSCs and abide by cGMP standards, thus allowing clinical applications, has not been described. We generated hiNSCs by a virus-free technique, whose properties recapitulate those of the clinical-grade hNSCs successfully used in an Amyotrophic Lateral Sclerosis (ALS) phase I clinical trial. Ex vivo, hiNSCs critically depend on exogenous mitogens for stable self-renewal and amplification and spontaneously differentiate into astrocytes, oligodendrocytes and neurons upon their removal. In the brain of immunodeficient mice, hiNSCs engraft and differentiate into neurons and glia, without tumour formation. These findings now warrant the establishment of clinical-grade, autologous and continuous hiNSC lines for clinical trials in neurological diseases such as Huntington’s, Parkinson’s and Alzheimer’s, among others.
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影响因子：
2.7
作者：
Hartfuss, E;Galli, R;Götz, M
通讯作者：
Götz, M
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A
影响因子：
2.5
作者：
LEVITT, P;RAKIC, P
通讯作者：
RAKIC, P
影响因子：
4.8
作者：
Baghbaderani, Behnam Ahmadian;Syama, Adhikarla;Rao, Mahendra S.
通讯作者：
Rao, Mahendra S.
DOI：
10.1016/j.omtm.2016.11.005
发表时间：
2017-03-17
期刊：
Molecular therapy. Methods & clinical development
影响因子：
--
作者：
Irion S;Zabierowski SE;Tomishima MJ
通讯作者：
Tomishima MJ