The response of retinal neurons to high-frequency stimulation.
The response of retinal neurons to high-frequency stimulation.
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DOI：
10.1088/1741-2560/10/3/036009
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发表时间：
2013-06
影响因子：
4
通讯作者：
Fried S
中科院分区：
文献类型：
--
作者：
Cai C;Twyford P;Fried S
高速率脉冲序列已被证明在人工耳蜗修复术中是有效的，最近，已被证明在PNS轴突中引起了广泛的有趣的反应特性。令人惊讶的是，这种训练在视网膜假体中的有效性还没有被探索过。采用细胞贴附膜片钳方法，测定了兔视网膜神经节细胞（RGC）类型off -快转瞬态（off -快转瞬态）和ON-OFF定向选择性（DS）对2000脉冲/秒（PPS）双相脉冲的体外反应。OFF-BT细胞反应开始于~ 20ua，最大反应发生在~ 40ua。有趣的是，峰值水平随着振幅的进一步增加而下降。相比之下，DS细胞在~25 uA时出现峰值，并随着刺激幅度的增加而保持强烈的峰值，即使在最高水平的测试中也是如此。因此，2000 PPS （~25 μA）的低振幅刺激对OFF-BT细胞有较强的激活作用，而对DS细胞的激活作用较弱。相反，高振幅（~75 μA）会强烈激活DS细胞，同时抑制OFF-BT细胞的反应。细胞类型之间的反应差异表明某些形式的优先激活可能是可能的，进一步的测试是必要的。此外，这里发现的反应差异的范围表明，激活机制比以前研究中描述的更为复杂。
High-rate pulse trains have proven to be effective in cochlear prosthetics and, more recently, have been shown to elicit a wide range of interesting response properties in axons of the PNS. Surprisingly, the effectiveness of such trains for use in retinal prostheses has not been explored. Using cell-attached patch clamp methods, we measured the in vitro response of two rabbit retinal ganglion cell (RGC) types, OFF-Brisk Transient (OFF-BT) and ON-OFF Directionally Selective (DS), to trains of biphasic pulses delivered at 2000 pulses per second (PPS). For OFF-BT cells, response onset occurred at ~20 uA, and maximum response occurred at ~40 uA. Interestingly, spiking levels decreased for further increases in amplitude. In contrast, DS cells had a spiking onset at ~25 uA and maintained strong spiking as stimulus amplitude was increased, even at the highest levels tested. Thus, a low-amplitude stimulus train at 2000 PPS (~25 μA) will activate OFF-BT cells strongly, while simultaneously activating DS cells only weakly. In contrast, a high amplitude train (~75 μA) will activate DS cells strongly while suppressing responses in OFF-BT cells. The response differences between cell types suggest some forms of preferential activation may be possible, and further testing is warranted. Further, the scope of the response differences found here suggests activation mechanisms that are more complex than those described in previous studies.
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影响因子：
1.8
作者：
Humayun, MS;Weiland, JD;de Juan, E
通讯作者：
de Juan, E
影响因子：
1.8
作者：
Cao, Xiwu;Merwine, David K.;Grzywacz, Norberto M.
通讯作者：
Grzywacz, Norberto M.
DOI：
10.1109/tnsre.2011.2163082
发表时间：
2011-10
期刊：
IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society
影响因子：
--
作者：
Joseph L;Butera RJ
通讯作者：
Butera RJ
DOI：
10.1007/bf01759722
发表时间：
1944-01-01
期刊：
PFLUGERS ARCH GES PHYSIOL
影响因子：
--
作者：
Gildemeister, Martin
通讯作者：
Gildemeister, Martin
影响因子：
4
作者：
Jensen, Ralph J.;Rizzo, Joseph F., III
通讯作者：
Rizzo, Joseph F., III