Laminar responses in the auditory cortex using a multielectrode array substrate for simultaneous stimulation and recording

Laminar responses in the auditory cortex using a multielectrode array substrate for simultaneous stimulation and recording
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使用多电极阵列基板同时刺激和记录听觉皮层的层状反应

DOI:
10.1002/tee.22810
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发表时间:
2018
影响因子:
1
通讯作者:
Tateno Takashi
Tateno Takashi
中科院分区:
工程技术4区
文献类型:
--
作者:
Takahashi Sota;Muramatsu Shuto;Nishikawa Jun;Satoh Kazuo;Murakami Shuichi;Tateno Takashi

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在这里,我们报告了多电极阵列(MEA)衬底的设计,制造和表征,用于体内同时刺激和记录。制造的衬底有两个锋利的柄,我们在其中安排了多个电极阵列,其中一个柄的表面积为50 × 50µm2,用于刺激(电极间距,150µm),另一个柄的表面积为15 × 15µm2,用于记录(电极间距,100µm)。我们的阻抗测量表明,电极值与硅基商用器件或略小尺寸电极器件的规定值很好地一致。电极表征后,对MEAs在小鼠听觉皮层的同步刺激和急性神经记录进行了评估。利用MEA基底,我们记录了短时局部电刺激驱动的时空局部场电位分布,特别是对小鼠听觉皮层的第4层。我们的研究结果表明,在体内使用该设备可以成功地同时进行刺激和记录。事实上,在不久的将来,该设备可能有助于理解由声音刺激和电微刺激驱动的局部网络活动的异同。©2018日本电气工程师学会。约翰·威利父子出版公司。
Here, we report the design, fabrication, and characterization of a multielectrode array (MEA) substrate for the use of simultaneous stimulation and recordingin vivo. The fabricated substrate has two sharp shanks in which we arranged multiple electrode arrays with surface areas of 50 × 50 µm2in one shank for stimulation (interelectrode interval, 150 µm) and 15 × 15 µm2in the other for recording (interelectrode interval, 100 µm). Our impedance measurements show that the electrode values are in good agreement with specified values for silicon‐based commercially available devices or slightly smaller‐sized electrode devices. After electrode characterization, the MEAs were evaluated for simultaneous stimulation and acute neural recording in the mouse auditory cortexin vivo. Using MEA substrates, we recorded spatiotemporal local field potential profiles driven by short local electrical stimulation, particularly to layer 4 of the mouse auditory cortex. Our results demonstrate successful simultaneous stimulation and recordings using the devicesin vivo. Indeed, the device may contribute to understanding similarities and differences in local network activity driven by sound stimulation and electric microstimulation in near future work. © 2018 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
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