The flavonoid compound apigenin prevents colonic inflammation and motor dysfunctions associated with high fat diet-induced obesity.
The flavonoid compound apigenin prevents colonic inflammation and motor dysfunctions associated with high fat diet-induced obesity.
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DOI：
10.1371/journal.pone.0195502
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Antonioli L
中科院分区：
文献类型：
--
作者：
Gentile D;Fornai M;Colucci R;Pellegrini C;Tirotta E;Benvenuti L;Segnani C;Ippolito C;Duranti E;Virdis A;Carpi S;Nieri P;Németh ZH;Pistelli L;Bernardini N;Blandizzi C;Antonioli L
芹菜素在预防肥胖方面具有一定的作用。然而，其对肥胖相关的肠运动功能障碍的假定作用尚不清楚。本研究在饮食诱导的肥胖小鼠模型中检测芹菜素对结肠炎性和运动异常的影响。雄性C57 BL/6 J小鼠用标准饮食（SD）或高脂饮食（HFD）喂养。SD或HFD小鼠用芹菜素（10 mg/Kg/天）处理。8周后，评估身体和附睾脂肪重量以及胆固醇、甘油三酯和葡萄糖水平。检测丙二醛（MDA）、IL-1β、IL-6水平及let-7 f表达。结肠嗜酸性粒细胞浸润，以及P物质（SP）和诱导型一氧化氮合酶（iNOS）的表达进行了评价。运动反应下引起的NOS和速激肽能收缩的封锁记录在体外从结肠纵肌制备。与SD小鼠相比，HFD动物显示体重、附睾脂肪重量和代谢指数增加。HFD小鼠显示结肠MDA、IL-1β和IL-6水平增加，以及结肠和附睾组织中let-7 f表达减少。HFD小鼠显示结肠嗜酸性粒细胞浸润增加。免疫组化显示HFD小鼠肌间神经节SP和iNOS表达增加。在HFD小鼠的制剂中，NOS阻断或由速激肽能刺激介导的电诱发收缩增强。芹菜素可对抗HFD小鼠体重和附睾脂肪重量的增加以及代谢指标的改变。芹菜素还降低MDA、IL-1β和IL-6水平以及嗜酸性粒细胞浸润、SP和iNOS表达，沿着电诱发的速激肽能和氮能收缩的正常化。此外，芹菜素使let-7 f在附睾脂肪组织中的表达正常化，但在结肠标本中没有。芹菜素预防全身代谢改变，对抗肠道炎症，使肥胖相关的结肠动力障碍正常化。
Apigenin can exert beneficial actions in the prevention of obesity. However, its putative action on obesity-associated bowel motor dysfunctions is unknown. This study examined the effects of apigenin on colonic inflammatory and motor abnormalities in a mouse model of diet-induced obesity. Male C57BL/6J mice were fed with standard diet (SD) or high-fat diet (HFD). SD or HFD mice were treated with apigenin (10 mg/Kg/day). After 8 weeks, body and epididymal fat weight, as well as cholesterol, triglycerides and glucose levels were evaluated. Malondialdehyde (MDA), IL-1β and IL-6 levels, and let-7f expression were also examined. Colonic infiltration by eosinophils, as well as substance P (SP) and inducible nitric oxide synthase (iNOS) expressions were evaluated. Motor responses elicited under blockade of NOS and tachykininergic contractions were recorded in vitro from colonic longitudinal muscle preparations. When compared to SD mice, HFD animals displayed increased body weight, epididymal fat weight and metabolic indexes. HFD mice showed increments in colonic MDA, IL-1β and IL-6 levels, as well as a decrease in let-7f expression in both colonic and epididymal tissues. HFD mice displayed an increase in colonic eosinophil infiltration. Immunohistochemistry revealed an increase in SP and iNOS expression in myenteric ganglia of HFD mice. In preparations from HFD mice, electrically evoked contractions upon NOS blockade or mediated by tachykininergic stimulation were enhanced. In HFD mice, Apigenin counteracted the increase in body and epididymal fat weight, as well as the alterations of metabolic indexes. Apigenin reduced also MDA, IL-1β and IL-6 colonic levels as well as eosinophil infiltration, SP and iNOS expression, along with a normalization of electrically evoked tachykininergic and nitrergic contractions. In addition, apigenin normalized let-7f expression in epididymal fat tissues, but not in colonic specimens. Apigenin prevents systemic metabolic alterations, counteracts enteric inflammation and normalizes colonic dysmotility associated with obesity.
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影响因子：
3.7
作者：
Kim KA;Gu W;Lee IA;Joh EH;Kim DH
通讯作者：
Kim DH
影响因子：
1.7
作者：
Della Vedova MC;Muñoz MD;Santillan LD;Plateo-Pignatari MG;Germanó MJ;Rinaldi Tosi ME;Garcia S;Gomez NN;Fornes MW;Gomez Mejiba SE;Ramirez DC
通讯作者：
Ramirez DC
影响因子：
4.3
作者：
Choi, Jae Sue;Islam, Md. Nurul;Jung, Hyun Ah
通讯作者：
Jung, Hyun Ah
影响因子：
2.9
作者：
Bouchoucha, Michel;Fysekidis, Marinos;Benamouzig, Robert
通讯作者：
Benamouzig, Robert
DOI：
10.1038/ijo.2015.170
发表时间：
2016-01
期刊：
International journal of obesity (2005)
影响因子：
--
作者：
Deiuliis JA
通讯作者：
Deiuliis JA