Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism.
Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism.
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DOI：
10.1371/journal.pone.0264337
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Agirbasli M
中科院分区：
文献类型：
--
作者：
Agirbasli D;Kalyoncu M;Muftuoglu M;Aksungar FB;Agirbasli M
维生素D缺乏在绝经后妇女中很常见。端粒长度可能是衰老相关疾病的潜在保护机制。我们研究的目的是检查维生素D补充与健康绝经后维生素D缺乏症妇女白细胞端粒长度（LTL）的关系。本研究设计为一项安慰剂对照研究，旨在研究补充维生素D和季节变化对绝经后妇女（n = 102）维生素D相关参数的短期影响，包括25(OH)D、1,25(OH)2D旁甲状旁激素（PTH）、维生素D结合蛋白（VDBP）、维生素D受体（VDR）和端粒长度。该组分为补充组（n = 52）和安慰剂组（n = 50）。治疗前后测量各项参数。采用ELISA法检测血清VDBP水平，采用实时荧光定量PCR法检测外周血单核细胞（PBMC） VDR、GC （VDBP）基因表达及相对端粒长度。结果表明，两组之间的基线水平相似。补充维生素D后，25(OH) D、1,25(OH)2D、PTH和VDBP水平与安慰剂组相比有显著变化。在研究期结束时，两组的LTL水平均显著升高，且安慰剂组的变化更为显著。治疗组和安慰剂组之间GC表达变化显著，但VDR表达保持不变。尽管这项研究的目的仅仅是评估补充维生素D的效果，但整个研究组的LTL在夏季都显著增加，这表明LTL水平受到阳光照射和季节变化的影响，而不是补充维生素D。本研究显示了补充维生素D对维生素D、PTH水平、LTL和维生素D相关基因表达的短期影响。维生素D与LTL之间的关系不是线性的，可能受到人群差异、地区和季节日照变化等多种因素的影响。
Vitamin D deficiency is common among postmenopausal women. Telomere length can be a potential protective mechanism for age-related diseases. The objective of our study is to examine the association of vitamin D supplementation on leukocyte telomere length (LTL) in healthy postmenopausal women with vitamin D deficiency. The study was designed as a placebo-controlled study to investigate the short-term effects of vitamin D supplementation and seasonal changes on vitamin D related parameters, including 25(OH)D, 1,25(OH)2D parathormone (PTH), Vitamin D binding protein (VDBP), vitamin D receptor (VDR), and telomere length in a cohort of postmenopausal women (n = 102). The group was divided as supplementation (n = 52) and placebo groups (n = 50). All parameters were measured before and after treatment. Serum VDBP levels were measured by ELISA method and VDR, GC (VDBP) gene expressions and relative telomere lengths were measured in peripheral blood mononuclear cells (PBMC) using a quantitative real-time PCR method. The results demonstrate that baseline levels were similar between the groups. After vitamin D supplementation 25(OH)D, 1,25(OH)2D, PTH and VDBP levels were changed significantly compared to the placebo group. At the end of the study period, LTL levels were significantly increased in both groups and this change was more prominent in placebo group. The change in GC expression was significant between treatment and placebo groups but VDR expression remained unchanged. Even though the study was designed to solely assess the effects of vitamin D supplementation, LTL was significantly increased in the whole study group in summer months suggesting that LTL levels are affected by sun exposure and seasonal changes rather than supplementation. The study displayed the short-term effect of Vitamin D supplementation on vitamin D, PTH levels, LTL and vitamin D associated gene expressions. The relation between Vitamin D and LTL is not linear and could be confounded by several factors such as the population differences, regional and seasonal changes in sun exposure.
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