Modification of female and male social behaviors in estrogen receptor beta knockout mice by neonatal maternal separation.
Modification of female and male social behaviors in estrogen receptor beta knockout mice by neonatal maternal separation.
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DOI：
10.3389/fnins.2014.00274
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发表时间：
2014
影响因子：
4.3
通讯作者：
Ogawa S
中科院分区：
文献类型：
--
作者：
Tsuda MC;Yamaguchi N;Nakata M;Ogawa S
母体分离是一种模拟早期生活应激对情绪和社会行为发展影响的动物模型。最近的研究表明，MS应激增加了雌性小鼠的社交焦虑水平，减少了雄性小鼠青春期前后的攻击性。雌激素受体(ER)β在应激反应、焦虑相关行为和社会行为的调节中起着关键作用。使用ERβ基因敲除(βERKO)小鼠的行为研究报告称，与野生型(WT)小鼠相比，βERKO雌性小鼠的社会调查增加，社交焦虑减少，βERKO雄性小鼠的攻击水平增加。在目前的研究中，使用βERKO和WT小鼠，我们检验了ERβ是否有助于MS对焦虑和社会行为的影响。β、ERKO和WT小鼠从出生后1-14天起每天(4h)离开DAM，对照组不受干扰。首先，在野外测试中，MS和ERβ基因缺失分别增加了焦虑相关行为，但仅限于雌性小鼠。在MS应激下，βERKO雌性小鼠的焦虑水平没有进一步改善。第二，在社会调查测试和长期社会偏好测试中，β尔科雌性小鼠的社会调查水平高于WT。然而，多发性硬化症大大缩短了社会调查持续时间，增加了WT和βErko女性在社会调查测试中的延伸途径数量，这表明这两种基因型的社交焦虑水平都有所提高。第三，青春期和成年βERKO雄性小鼠比WT小鼠更具攻击性，这表明攻击持续时间更长。另一方面，MS显著缩短了两种基因型鼠的攻击持续时间，但仅限于青春期前后的雄性小鼠。综上所述，这些结果表明，βERKO小鼠对MS应激对随后的雌性和雄性社会行为的不利影响非常敏感，这可能会超过ERβ对雌性社交焦虑和雄性攻击性的影响。
Maternal separation (MS) is an animal model mimicking the effects of early life stress on the development of emotional and social behaviors. Recent studies revealed that MS stress increased social anxiety levels in female mice and reduced peri-pubertal aggression in male mice. Estrogen receptor (ER) β plays a pivotal role in the regulation of stress responses and anxiety-related and social behaviors. Behavioral studies using ERβ knockout (βERKO) mice reported increased social investigation and decreased social anxiety in βERKO females, and elevated aggression levels in βERKO males compared to wild-type (WT) mice. In the present study, using βERKO and WT mice, we examined whether ERβ contributes to MS effects on anxiety and social behaviors. βERKO and WT mice were separated from their dam daily (4 h) from postnatal day 1–14 and control groups were left undisturbed. First, MS and ERβ gene deletion individually increased anxiety-related behaviors in the open field test, but only in female mice. Anxiety levels were not further modified in βERKO female mice subjected to MS stress. Second, βERKO female mice showed higher levels of social investigation compared with WT in the social investigation test and long-term social preference test. However, MS greatly reduced social investigation duration and elevated number of stretched approaches in WT and βERKO females in the social investigation test, suggesting elevated levels of social anxiety in both genotypes. Third, peri-pubertal and adult βERKO male mice were more aggressive than WT mice as indicated by heightened aggression duration. On the other hand, MS significantly decreased aggression duration in both genotypes, but only in peri-pubertal male mice. Altogether, these results suggest that βERKO mice are sensitive to the adverse effects of MS stress on subsequent female and male social behaviors, which could then have overrode the ERβ effects on female social anxiety and male aggression.
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影响因子：
3.2
作者：
Handa RJ;Ogawa S;Wang JM;Herbison AE
通讯作者：
Herbison AE
影响因子：
--
作者：
CRITCHLOW, V;BARSELA, M;LIPSCOMB, HS
通讯作者：
LIPSCOMB, HS
影响因子：
2.2
作者：
Hohmann, Christine F.;Hodges, Amber;Aneni, Justin
通讯作者：
Aneni, Justin
影响因子：
2.5
作者：
Merchenthaler, I;Lane, MV;Dellovade, TL
通讯作者：
Dellovade, TL
影响因子：
4.8
作者：
Lund, TD;Rovis, T;Handa, RJ
通讯作者：
Handa, RJ