Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration.
Dentin-Derived Inorganic Minerals Promote the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Applications for Bone Regeneration.
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牙本质源性无机矿物质促进骨髓源性间充质干细胞的成骨作用：骨再生的潜在应用
DOI：
10.1155/2020/8889731
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发表时间：
2020
影响因子：
4.3
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Lei G;Wang Y;Yu Y;Li Z;Lu J;Ge X;Li N;Manduca AGC;Yu J
背景口腔颌面部骨缺损在人群中非常普遍，如今，越来越多的注意力集中在牙本质衍生物作为理想的骨再生移植材料上。本研究采用高温煅烧法制备牙本质无机矿物材料（DIM），探讨DIM对骨髓间充质干细胞（BMMSCs）成骨分化及骨形成的影响。方法采用CCK-8法、流式细胞术和EdU法检测DIM对BMMSC增殖和凋亡的影响。通过碱性磷酸酶（ALP）活性检测、ALP染色、茜素红染色及成骨标志物表达分析，探讨DIM对BMMSCs成骨分化的影响及其信号机制。建立大鼠颅骨临界缺损模型，探讨DIM对骨再生的体内效应。结果细胞活力测定表明DIM无细胞毒性。与对照组相比，DIM培养的BMMSCs具有更高的成骨分化能力。在DIM处理的BMMSCs中检测到ERK和p38信号的激活，并且ERK抑制剂U 0126和p38抑制剂SB 203580分别抑制了这两条途径和成骨过程。此外，动物实验表明，与对照组相比，DIM可以显着促进新骨形成。结论DIM可通过激活ERK和p38 MAPK信号通路促进BMMSC向成骨细胞分化，有望成为一种新型的促骨形成材料。
Background Oral and maxillofacial bone loss is highly prevalent among populations, and nowadays, increased attention has been focused on dentin derivatives serving as desirable graft materials for bone regeneration. In this study, dentin-derived inorganic mineral (DIM) was fabricated with a high-temperature calcination technique and the effects of DIM on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMMSCs) and the bone formation were elucidated. Methods The effects of DIM on BMMSC proliferation and apoptosis capacity were evaluated by CCK-8, flow cytometry, and EdU assays. Alkaline phosphatase (ALP) activity detection, ALP staining, alizarin red staining, and osteogenic marker expression analysis were performed to investigate the influence of DIM on the osteogenic differentiation of BMMSCs, as well as the relevant signal mechanisms. The model of critical-sized defects in the calvarium of rats was constructed for exploring the in vivo efficiency of DIM on bone regeneration. Results Cell viability assays indicated that DIM had no cytotoxicity. BMMSCs cultured with DIM presented a higher level of osteogenic differentiation ability than those in the control group. The activation in ERK and p38 signals was detected in DIM-treated BMMSCs, and both pathways and osteogenic process were suppressed while using ERK inhibitor U0126 and p38 inhibitor SB203580, respectively. Furthermore, the animal experiments revealed that DIM could dramatically enhance new bone formation compared to the control group. Conclusion DIM could promote BMMSC osteogenic differentiation via triggering the ERK and p38 MAPK signaling pathways and might be a novel predictable material for facilitating bone formation.
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DOI：
10.5125/jkaoms.2013.39.3.103
发表时间：
2013-06
影响因子：
1
作者：
Kim YK;Lee J;Um IW;Kim KW;Murata M;Akazawa T;Mitsugi M
通讯作者：
Mitsugi M
间歇性施用甲状旁腺激素通过 JNK 和 P38 MAPK 途径增强根尖乳头干细胞的牙向/成骨分化
影响因子：
4.3
作者：
Pang, Xiyao;Zhuang, Ying;Yu, Jinhua
通讯作者：
Yu, Jinhua
影响因子：
3.6
作者：
Kamalaldin, Nurulain 'Atikah;Jaafar, Mariatti;Yahaya, Badrul Hisham
通讯作者：
Yahaya, Badrul Hisham
影响因子：
4.6
作者：
Endale M;Kim TH;Kwak YS;Kim NM;Kim SH;Cho JY;Yun BS;Rhee MH
通讯作者：
Rhee MH
CaMKII 参与镉激活 MAPK 和 mTOR 通路，导致神经元细胞死亡
影响因子：
4.7
作者：
Chen S;Xu Y;Xu B;Guo M;Zhang Z;Liu L;Ma H;Chen Z;Luo Y;Huang S;Chen L
通讯作者：
Chen L