Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus.
Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus.
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DOI：
10.1186/1477-7827-10-71
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发表时间：
2012-09-05
期刊：
影响因子：
--
通讯作者：
Matsuyama M
中科院分区：
文献类型：
--
作者：
Ohga H;Kaneko K;Shimizu A;Kitano H;Selvaraj S;Nyuji M;Adachi H;Yamaguchi A;Matsuyama M
促性腺激素 (GtH)、卵泡刺激素 (FSH) 和黄体生成素 (LH) 在垂体中产生，并通过性腺类固醇的产生调节配子发生。然而，由于纯化天然 GtH 的技术困难，两种 GtH 在硬骨鱼中各自的作用仍不完全表征。通过阴离子交换层析和使用特异性抗血清的免疫印迹从成年鲭鱼、鲭鱼的垂体中纯化天然 FSH 和 LH。通过测量性腺类固醇、雌二醇-17β (E2) 和 17,20β-二羟基-4-孕烯-3-酮 (17,20β-P) 的水平，评估了卵黄发生中期和晚期卵泡中完整鲭鱼 FSH (cmFSH) 和 LH (cmLH) 的类固醇生成效力。此外，我们还评估了 GtH 对同一阶段卵泡的成熟诱导能力。 cmFSH 和 cmLH 均显着刺激卵黄发生中期卵泡中 E2 的产生。相比之下，只有 LH 显着刺激卵黄发生晚期卵泡中 17,20β-P 的产生。同样，cmLH 在卵黄发生晚期卵泡中诱导最终卵母细胞成熟 (FOM)。目前的结果表明，FSH 和 LH 都可以调节卵黄形成过程，而只有 LH 启动鲭鱼的 FOM。
The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs. Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles. Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles. Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.
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影响因子：
2.7
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