BASI74, a Virulence-Related sRNA in Brucella abortus.
BASI74, a Virulence-Related sRNA in Brucella abortus.
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BASI74，流产布鲁氏菌毒力相关的 sRNA。
DOI：
10.3389/fmicb.2018.02173
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wu Q
中科院分区：
文献类型：
--
作者：
Dong H;Peng X;Liu Y;Wu T;Wang X;De Y;Han T;Yuan L;Ding J;Wang C;Wu Q
布鲁氏菌是感染包括人类在内的多种哺乳动物的细胞内病原体，对发展中国家的畜牧业和人类健康构成威胁。迄今为止，在布鲁氏菌中已经鉴定出许多与细胞内运输和增殖相关的基因。然而，复杂的转录后调控和基因表达的协调，使布鲁氏菌。以适应环境的变化和逃避宿主细胞的防御还没有完全理解。细菌小RNA（sRNAs）在转录后调控中发挥重要作用，这已经在许多细菌中得到证实，但是sRNAs在布鲁氏菌中的作用仍然是难以捉摸的。在这项研究中，我们在布鲁氏菌中鉴定了几种不同的sRNA，并发现一种暂时命名为BASI 74的sRNA的过表达导致布鲁氏菌在巨噬细胞感染模型中毒力的改变。BASI 74的表达水平随着布鲁氏菌2308在酸性培养基中的生长而增加。此外，BASI 74还影响布鲁菌在基本培养基和铁限制培养基中的生长率。使用双质粒报告系统，我们确定了四个基因作为BASI 74的目标。一个靶基因BABI 1154被预测编码胞嘧啶-N4特异性DNA甲基转移酶，其保护细胞DNA免受布鲁氏菌中限制性内切酶的侵害。这些结果表明，BASI 74在巨噬细胞感染模型中的布鲁氏菌存活中起重要作用，推测其与应激反应或对限制修饰系统的影响有关。我们的研究促进了对布鲁氏菌sRNA的理解，以及sRNA用于影响布鲁氏菌生理和发病机制的机制。
Brucella spp. are intracellular pathogens that infect a wide variety of mammals including humans, posing threats to the livestock industry and human health in developing countries. A number of genes associated with the intracellular trafficking and multiplication have so far been identified in Brucella spp. However, the sophisticated post-transcriptional regulation and coordination of gene expression that enable Brucella spp. to adapt to changes in environment and to evade host cell defenses are not fully understood. Bacteria small RNAs (sRNAs) play a significant role in post-transcriptional regulation, which has already been confirmed in a number of bacteria but the role of sRNAs in Brucella remains elusive. In this study, we identified several different sRNAs in Brucella spp., and found that over-expression of a sRNA, tentatively termed BASI74, led to alternation in virulence of Brucella in macrophage infection model. The expression level of BASI74 increased while Brucella abortus 2308 was grown in acidic media. In addition, BASI74 affected the growth ratio of the Brucella cells in minimal media and iron limiting medium. Using a two-plasmid reporter system, we identified four genes as the target of BASI74. One target gene, BABI1154, was predicted to encode a cytosine-N4-specific DNA methyltransferase, which protects cellular DNA from the restriction endonuclease in Brucella. These results show that BASI74 plays an important role in Brucella survival in macrophage infection model, speculatively by its connection with stress response or impact on restriction-modification system. Our study promotes the understanding of Brucella sRNAs, as well as the mechanism by which sRNAs use to influence Brucella physiology and pathogenesis.
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鉴定调节布鲁氏菌细胞内存活的新型小非编码 RNA。
影响因子：
5.2
作者：
Wang Y;Ke Y;Xu J;Wang L;Wang T;Liang H;Zhang W;Gong C;Yuan J;Zhuang Y;An C;Lei S;Du X;Wang Z;Li W;Yuan X;Huang L;Yang X;Chen Z
通讯作者：
Chen Z
影响因子：
64.5
作者：
Waters LS;Storz G
通讯作者：
Storz G
影响因子：
5.4
作者：
Roop RM 2nd;Gaines JM;Anderson ES;Caswell CC;Martin DW
通讯作者：
Martin DW
影响因子：
3.1
作者：
Abdou, Elias;Deredjian, Amelie;Jubier-Maurin, Veronique
通讯作者：
Jubier-Maurin, Veronique
影响因子：
14.9
作者：
Wright PR;Georg J;Mann M;Sorescu DA;Richter AS;Lott S;Kleinkauf R;Hess WR;Backofen R
通讯作者：
Backofen R