Protocol for controlling visual experience during zebrafish development and modulation of motor behavior.
Protocol for controlling visual experience during zebrafish development and modulation of motor behavior.
复制标题
控制斑马鱼发育期间的视觉体验和运动行为调节的协议。
DOI:
10.1016/j.xpro.2023.102636
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发表时间:
2023-12-15
期刊:
影响因子:
--
通讯作者:
Horstick, Eric J.
中科院分区:
文献类型:
--
作者:
Hageter, John;Starkey, Jacob;Barr, Allison;Huff, Johnathon R.;Horstick, Eric J.
Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023). Protocol for controlling visual experience to one or both eyes Visual control can be performed during different developmental windows Flexible behavioral rig for recording photo-mediated larval zebrafish behavior Straightforward programs for data analysis to describe zebrafish movement Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity.
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影响因子:
3
作者:
Hageter J;Waalkes M;Starkey J;Copeland H;Price H;Bays L;Showman C;Laverty S;Bergeron SA;Horstick EJ
通讯作者:
Horstick EJ
影响因子:
4.6
作者:
Xie, Jiaheng;Jusuf, Patricia R.;Goodbourn, Patrick T.
通讯作者:
Goodbourn, Patrick T.
影响因子:
2.5
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McArthur,Kimberly L.;Tovar,Victoria M.;Astad,Emma K.
通讯作者:
Astad,Emma K.
影响因子:
5.4
作者:
Horstick EJ;Bayleyen Y;Sinclair JL;Burgess HA
通讯作者:
Burgess HA
影响因子:
16.6
作者:
Sherman, Shachar;Arnold-Ammer, Irene;Schneider, Martin W.;Kawakami, Koichi;Baier, Herwig
通讯作者:
Baier, Herwig