Protocol for controlling visual experience during zebrafish development and modulation of motor behavior.

Protocol for controlling visual experience during zebrafish development and modulation of motor behavior.
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控制斑马鱼发育期间的视觉体验和运动行为调节的协议。

DOI:
10.1016/j.xpro.2023.102636
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发表时间:
2023-12-15
期刊:
影响因子:
--
通讯作者:
Horstick, Eric J.
Horstick, Eric J.
中科院分区:
其他
文献类型:
--
作者:
Hageter, John;Starkey, Jacob;Barr, Allison;Huff, Johnathon R.;Horstick, Eric J.

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感觉经验指导神经发育并改善感觉处理。在这里,我们描述了一种微创协议,以控制视觉体验的早期发展过程中,斑马鱼。我们描述了如何准备幼虫包埋在琼脂糖在两个不同的时间点发展。然后,我们描述了如何建立一个行为钻机和使用软件来跟踪斑马鱼的行为。最后,我们详细分析了行为数据,以验证该协议,并确定感官依赖可塑性的结果。有关本方案使用和执行的完整详细信息,请参见Hageter et al.(2023年)。控制单眼或双眼视觉体验的方案视觉控制可以在不同的发育窗口期间进行灵活的行为装置用于记录光介导的斑马鱼幼虫行为用于描述斑马鱼运动的数据分析的简单程序出版商说明:进行任何实验方案都需要遵守当地机构的实验室安全和道德准则。感觉经验指导神经发育并改善感觉处理。在这里,我们描述了一种微创协议,以控制视觉体验的早期发展过程中,斑马鱼。我们描述了如何准备幼虫包埋在琼脂糖在两个不同的时间点发展。然后,我们描述了如何建立一个行为钻机和使用软件来跟踪斑马鱼的行为。最后,我们详细分析了行为数据,以验证该协议,并确定感官依赖可塑性的结果。
Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity. For complete details on the use and execution of this protocol, please refer to Hageter et al. (2023). Protocol for controlling visual experience to one or both eyes Visual control can be performed during different developmental windows Flexible behavioral rig for recording photo-mediated larval zebrafish behavior Straightforward programs for data analysis to describe zebrafish movement Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Sensory experience instructs neurodevelopment and refines sensory processing. Here, we describe a minimally invasive protocol to immobilize zebrafish during early development to control visual experience. We describe how to prepare larvae for embedding in agarose at two separate timepoints in development. Then we describe how to build a behavior rig and use software to track zebrafish behaviors. Finally, we detail analyzing behavioral data to validate the protocol and determine outcomes of sensory dependent plasticity.
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