Retina-derived signals control pace of neurogenesis in visual brain areas but not circuit assembly.
Retina-derived signals control pace of neurogenesis in visual brain areas but not circuit assembly.
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DOI：
10.1038/s41467-023-40749-1
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发表时间：
2023-09-27
影响因子：
16.6
通讯作者：
Baier, Herwig
中科院分区：
文献类型：
--
作者：
Sherman, Shachar;Arnold-Ammer, Irene;Schneider, Martin W.;Kawakami, Koichi;Baier, Herwig
大脑发育是由先天和经验依赖机制协调的，但它们的相对贡献很难理清。在这里，我们问是否以及如何改变中央视觉区在脊椎动物大脑耗尽任何和所有信号从视网膜神经节细胞在整个发展。我们转录分析神经元在前顶盖，丘脑和其他视网膜受体领域的斑马鱼幼虫，并寻找缺乏所有视网膜连接的lakritz突变体的变化。虽然个别基因失调，但77种神经元类型的完整集合以明显正常的比例、在正常的位置和沿着正常的分化轨迹发育。引人注目的是，在这些领域的增殖祖细胞的细胞周期出口被延迟，和早期有丝分裂后的前体细胞的更大比例保持未提交或被转移到前胶质细胞的命运。针对通常参与处理视觉信息的神经元组的光遗传刺激引起与野生型无区别的行为。总之，我们表明，视网膜轴突发出的信号影响视觉脑区神经发生的步伐，但不会明显影响下游神经元的规格或布线。在发育过程中，感觉输入如何塑造大脑的细胞组成和电路结构？这项研究表明，如果没有与视网膜的连接，大脑视觉区域的神经元会正常分化和连接，尽管速度较慢。
Brain development is orchestrated by both innate and experience-dependent mechanisms, but their relative contributions are difficult to disentangle. Here we asked if and how central visual areas are altered in a vertebrate brain depleted of any and all signals from retinal ganglion cells throughout development. We transcriptionally profiled neurons in pretectum, thalamus and other retinorecipient areas of larval zebrafish and searched for changes in lakritz mutants that lack all retinal connections. Although individual genes are dysregulated, the complete set of 77 neuronal types develops in apparently normal proportions, at normal locations, and along normal differentiation trajectories. Strikingly, the cell-cycle exits of proliferating progenitors in these areas are delayed, and a greater fraction of early postmitotic precursors remain uncommitted or are diverted to a pre-glial fate. Optogenetic stimulation targeting groups of neurons normally involved in processing visual information evokes behaviors indistinguishable from wildtype. In conclusion, we show that signals emitted by retinal axons influence the pace of neurogenesis in visual brain areas, but do not detectably affect the specification or wiring of downstream neurons. How does sensory input shape cellular composition and circuit structure of the brain during development? This study shows that, without connections to the retina, neurons in visual brain areas differentiate and wire up normally, albeit more slowly.
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影响因子：
64.5
作者：
Cheng S;Butrus S;Tan L;Xu R;Sagireddy S;Trachtenberg JT;Shekhar K;Zipursky SL
通讯作者：
Zipursky SL
影响因子：
16.2
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通讯作者：
Kharchenko PV
影响因子：
48
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Lange M;Bergen V;Klein M;Setty M;Reuter B;Bakhti M;Lickert H;Ansari M;Schniering J;Schiller HB;Pe'er D;Theis FJ
通讯作者：
Theis FJ
影响因子：
64.8
作者：
Belluscio, L;Lodovichi, C;Katz, LC
通讯作者：
Katz, LC