Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA.

Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA.
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通过对植物皮质RNA的微阵列分析来鉴定种植血浆相关的转录本。

DOI:
10.1002/dvdy.22304
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发表时间:
2010-06
影响因子:
2.5
通讯作者:
Houston, Douglas W.
Houston, Douglas W.
中科院分区:
生物学3区
文献类型:
--
作者:
Cuykendall, Tawny N.;Houston, Douglas W.

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RNA定位是调节细胞结构和功能的常见机制。非洲爪蟾卵母细胞中的局部RNA对于早期发育至关重要,包括由种质进行的种系特化。尽管这些定位的RNA的重要性,只有大约25已被确定,更少的功能特性。使用微阵列,我们确定了一个大型的本地化RNA从植物皮层。总的来说,我们的研究结果表明,在卵母细胞中至少有275个本地化的RNA,或2-3%的母体转录本,这与以前的研究结果基本一致。我们进一步验证了24个候选人的植物定位,并进一步表征了种质中表达的三个基因。我们确定了新的种质表达reticulon 3.1,exd 2(一种新的核酸外切酶结构域编码基因),和一个假定的非编码RNA。对这些和其他定位RNA的进一步分析将可能鉴定种质的新功能,并促进顺式作用RNA定位元件的鉴定。
RNA localization is a common mechanism for regulating cell structure and function. Localized RNAs in Xenopus oocytes are critical for early development, including germline specification by the germ plasm. Despite the importance of these localized RNAs, only approximately 25 have been identified and fewer are functionally characterized. Using microarrays, we identified a large set of localized RNAs from the vegetal cortex. Overall, our results indicate a minimum of 275 localized RNAs in oocytes, or 2–3% of maternal transcripts, which are in general agreement with previous findings. We further validated vegetal localization for 24 candidates and further characterized three genes expressed in the germ plasm. We identified novel germ plasm expression for reticulon 3.1, exd2 (a novel exonuclease-domain encoding gene), and a putative noncoding RNA. Further analysis of these and other localized RNAs will likely identify new functions of germ plasm and facilitate the identification of cis-acting RNA localization elements.
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