Stereotaxic Exposure of the Central Nucleus of the Amygdala to Corticosterone Increases Colonic Permeability and Reduces Nerve-Mediated Active Ion Transport in Rats.
Stereotaxic Exposure of the Central Nucleus of the Amygdala to Corticosterone Increases Colonic Permeability and Reduces Nerve-Mediated Active Ion Transport in Rats.
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DOI：
10.3389/fnins.2018.00543
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发表时间：
2018
影响因子：
4.3
通讯作者：
Greenwood-Van Meerveld B
中科院分区：
文献类型：
--
作者：
Hattay P;Prusator DK;Johnson AC;Greenwood-Van Meerveld B
背景：肠易激综合征（IBS）的特征是内脏疼痛和异常的排便习惯，这些症状在压力下会恶化。证据还表明IBS的肠屏障功能改变。以前，我们证明，立体定向应用应激激素皮质酮（CORT）到中央杏仁核（CeA）诱导结肠痛觉过敏和焦虑样行为的大鼠模型，但对肠通透性和粘膜功能的影响仍有待评估。研究方法：雄性Fischer 344大鼠接受双侧立体定位植入CORT或含惰性胆固醇（CHOL）的微丸（30 μg）到CeA的背侧边缘。7天后，分离结肠组织以通过跨上皮电阻（TEER）和辣根过氧化物酶（HRP）的大分子通量评估改良的Ussing室中的组织渗透性。粘膜下肠神经对电场刺激（EFS）的分泌反应以及用毛喉素激活被用来评估离子在分离的结肠组织中的运动。在一个单独的队列中，对结肠组织学和肥大细胞浸润进行了评估。关键成果：与植入CHOL的对照组相比，我们确定将CeA暴露于升高水平的CORT显著增加了穿过结肠上皮层的大分子通量，而不改变TEER。杏仁核CORT升高可抑制神经介导的主动转运，但不抑制cAMP介导的主动转运。CORT处理动物的结肠组织内没有组织学变化或肥大细胞浸润增加。结论和推论：这些观察结果支持CeA作为神经介导的结肠上皮功能的调节剂的新作用。
Background: Irritable bowel syndrome (IBS) is characterized by visceral pain and abnormal bowel habits that are worsened during stress. Evidence also suggests altered intestinal barrier function in IBS. Previously, we demonstrated that stereotaxic application of the stress hormone corticosterone (CORT) onto the central nucleus of the amygdala (CeA) induces colonic hyperalgesia and anxiety-like behavior in a rat model, however the effect on intestinal permeability and mucosal function remain to be evaluated. Methods: Male Fischer 344 rats underwent bilateral stereotaxic implantation of CORT or inert cholesterol (CHOL)-containing micropellets (30 μg) onto the dorsal margin of the CeA. Seven days later, colonic tissue was isolated to assess tissue permeability in modified Ussing chambers via transepithelial electrical resistance (TEER) and macromolecular flux of horseradish peroxidase (HRP). Secretory responses to electrical field stimulation (EFS) of submucosal enteric nerves as well as activation with forskolin were used to assess movements of ions across the isolated colonic tissues. In a separate cohort, colonic histology, and mast cell infiltration was assessed. Key Results: Compared to CHOL-implanted controls, we determined that exposing the CeA to elevated levels of CORT significantly increased macromolecular flux across the colonic epithelial layer without changing TEER. Nerve-mediated but not cAMP-mediated active transport was inhibited in response to elevated amygdala CORT. There were no histological changes or increases in mast cell infiltration within colonic tissue from CORT treated animals. Conclusion and Inferences: These observations support a novel role for the CeA as a modulator of nerve-mediated colonic epithelial function.
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影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
DOI：
10.1016/j.nicl.2017.06.001
发表时间：
2017
期刊：
NeuroImage. Clinical
影响因子：
--
作者：
Icenhour A;Witt ST;Elsenbruch S;Lowén M;Engström M;Tillisch K;Mayer EA;Walter S
通讯作者：
Walter S
DOI：
10.1093/gerona/glt106
发表时间：
2013-09-01
影响因子：
5.1
作者：
Lee Tran;Greenwood-Van Meerveld, Beverley
通讯作者：
Greenwood-Van Meerveld, Beverley
影响因子：
2.9
作者：
Greenwood-Van Meerveld, B;Gibson, M;Myers, D
通讯作者：
Myers, D
影响因子：
3.5
作者：
Fuentes IM;Christianson JA
通讯作者：
Christianson JA