Lactic acid bacteria exhibit similar antioxidant capacities in Caenorhabditis elegans- and Campylobacter jejuni-infected mice.
Lactic acid bacteria exhibit similar antioxidant capacities in Caenorhabditis elegans- and Campylobacter jejuni-infected mice.
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乳酸菌在秀丽隐杆线虫和空肠弯曲杆菌感染的小鼠中表现出相似的抗氧化能力
DOI：
10.1039/c9ra06105c
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发表时间：
2020-01-16
期刊：
影响因子：
3.9
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Jin, Xing;He, Yufeng;Liu, Zhenmin;Zhou, Yonghua;Chen, Xiaohua;Wang, Gang;Sun, Zhihong;Zhao, Jianxin;Zhang, Hao;Chen, Wei
秀丽隐杆线虫（C. elegans）越来越多地用作模型生物来筛选益生菌以进行衰老研究。在本研究中，使用暴露于 H2O2 和胡桃醌（用于慢性和急性测定）的线虫寿命分析模型来预选具有抗氧化应激能力的乳酸菌 (LAB)。分析了氧化应激的保护机制。筛选的 10 个 LAB 菌株中有 7 个（即 427、X13、9-5、422、Z5、G14 和 H29M-8M）对秀丽隐杆线虫表现出更高水平的保护，与非活性菌株 408、13-7 和 430（35% 至 45% 存活率）相比，存活率范围为 70% 至 85%。七种氧化应激耐受菌株的存活率与线虫在 H2O2 和胡桃醌暴露期间过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD) 和丙二醛 (MDA) 水平的增加以及活性氧 (ROS) 表达的减少相关。此外，丝裂原激活蛋白激酶（MAPK）和Nrf2-Keap1-ARE通路相关基因的转录在LAB分离株的氧化应激保护中升高。最后，发现这些 LAB 菌株保护线虫免受 H2O2 或胡桃酮暴露的能力与其在空肠弯曲杆菌感染小鼠中的抗氧化能力之间存在显着相关性。因此，结果表明，基于氧化应激的线虫寿命模型可用于在病原体感染的哺乳动物中筛选具有抗氧化功效的LAB。
Caenorhabditis elegans (C. elegans) is increasingly used as a model organism to screen probiotics for ageing research. In this study, a C. elegans lifespan analysis model exposed to H2O2 and juglone (for chronic and acute assays) was used to preselect lactic acid bacteria (LAB) with resistance to oxidative stress. The mechanism of oxidative stress protection was analyzed. Seven out of ten LAB strains screened for namely 427, X13, 9-5, 422, Z5, G14 and H29M-8M demonstrated higher levels of protection to C. elegans, ranging from 70% to 85% survival rate, in comparison to the inactive strains 408, 13-7 and 430 (35% to 45% survival rate). The survival rates of the seven oxidative stress tolerant strains were correlated to increase in catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA) levels and decrease in reactive oxygen species (ROS) expression in C. elegans during H2O2 and juglone exposure. In addition, the transcription of mitogen-activated protein kinase (MAPK) and Nrf2-Keap1-ARE pathway related genes was elevated in the oxidative stress protection of LAB isolates. Finally, a significant correlation was found between the ability of these LAB strains to protect C. elegans from H2O2 or juglone exposure and their antioxidative ability in Campylobacter jejuni-infected mice. Thus the results indicated that the oxidative stress-based lifespan model of C. elegans is useful for screening of LAB with antioxidant efficacy in pathogen-infected mammals.
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影响因子：
4.6
作者：
Lee IT;Yang CM
通讯作者：
Yang CM
影响因子：
8.8
作者：
Jones RM;Desai C;Darby TM;Luo L;Wolfarth AA;Scharer CD;Ardita CS;Reedy AR;Keebaugh ES;Neish AS
通讯作者：
Neish AS
影响因子：
5.3
作者：
Kobayashi, Makoto;Li, Li;Yamamoto, Masayuki
通讯作者：
Yamamoto, Masayuki
DOI：
10.1098/rstb.2010.0276
发表时间：
2011-01-12
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Kenyon C
通讯作者：
Kenyon C
影响因子：
4.8
作者：
Ho, YS;Xiong, Y;Ho, DS
通讯作者：
Ho, DS