Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans.
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans.
复制标题
DOI：
10.1038/s41598-021-86427-4
复制
发表时间：
2021-03-25
影响因子：
4.6
通讯作者：
Cuevas JM
中科院分区：
文献类型：
--
作者：
Cebriá-Mendoza M;Arbona C;Larrea L;Díaz W;Arnau V;Peña C;Bou JV;Sanjuán R;Cuevas JM
人类血液元基因组学揭示了在看似健康的受试者中存在不同类型的病毒。到目前为止，阿涅罗病毒构成了在人类血液中更常见的病毒家族，尽管扩增偏差和污染构成了这一领域的主要挑战。为了进一步研究这一点，我们对来自西班牙的120名健康捐赠者的混合血浆样本进行了高速离心法、RNA和DNA提取、随机扩增和大规模平行测序。我们的结果证实了在这类样本中广泛存在的阿涅罗病毒，占获得的总病毒序列的近97%。我们组装了属于这个家族的114个不同的病毒基因组，揭示了惊人的多样性。ORF1的系统发育分析提出了28个潜在的新Anellov物种，其中24个通过Sanger测序进行了验证，以丢弃人工制品。这些发现强调了实施更有效的提纯程序以丰富病毒部分的重要性，这是病毒体研究的关键步骤，并对阿涅罗病毒的病理作用提出了质疑。
Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this field. To investigate this further, we subjected pooled plasma samples from 120 healthy donors in Spain to high-speed centrifugation, RNA and DNA extraction, random amplification, and massive parallel sequencing. Our results confirm the extensive presence of anelloviruses in such samples, which represented nearly 97% of the total viral sequence reads obtained. We assembled 114 different viral genomes belonging to this family, revealing remarkable diversity. Phylogenetic analysis of ORF1 suggested 28 potentially novel anellovirus species, 24 of which were validated by Sanger sequencing to discard artifacts. These findings underscore the importance of implementing more efficient purification procedures that enrich the viral fraction as an essential step in virome studies and question the suggested pathological role of anelloviruses.
登录
查看更多内容
影响因子：
9.4
作者：
Cheval, Justine;Sauvage, Virginie;Eloit, Marc
通讯作者：
Eloit, Marc
影响因子：
4.6
作者：
Eibach, Daniel;Hogan, Benedikt;Cadar, Daniel
通讯作者：
Cadar, Daniel
DOI：
10.1146/annurev-virology-031413-085540
发表时间：
2015-01-01
期刊：
ANNUAL REVIEW OF VIROLOGY, VOL 2
影响因子：
--
作者：
Chow, Cheryl-Emiliane T.;Suttle, Curtis A.
通讯作者：
Suttle, Curtis A.
影响因子：
3.7
作者：
Hrazdilova, Kristyna;Slaninkova, Eva;Celer, Vladimir
通讯作者：
Celer, Vladimir
影响因子：
3.7
作者：
Gonzales-Gustavson E;Timoneda N;Fernandez-Cassi X;Caballero A;Abril JF;Buti M;Rodriguez-Frias F;Girones R
通讯作者：
Girones R