Microarray analysis of Pseudomonas aeruginosa reveals induction of pyocin genes in response to hydrogen peroxide.
Microarray analysis of Pseudomonas aeruginosa reveals induction of pyocin genes in response to hydrogen peroxide.
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DOI：
10.1186/1471-2164-6-115
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发表时间：
2005-09-08
期刊：
影响因子：
4.4
通讯作者：
Bentley WE
中科院分区：
文献类型：
--
作者：
Chang W;Small DA;Toghrol F;Bentley WE
铜绿假单胞菌是一种感染囊性纤维化患者的病原体，在活动性感染期间会遇到包括过氧化氢在内的吞噬细胞衍生的活性氧化剂的毒性。铜绿假单胞菌对这些有毒物种采取适应性和保护性策略，有效地感染人类。尽管我们在许多特定情况下对氧化应激反应的理解取得了进展，但靶向保护基因和细胞代谢的其余部分之间的联系仍然不清楚。在这里，我们对细胞对过氧化氢的反应进行了全基因组转录组学分析，以确定氧化应激诱导的基因是如何相关和调控的更完整的图景。我们的数据证实了先前的结论，即DNA修复蛋白和过氧化氢酶可能是铜绿假单胞菌最重要的抗氧化防御系统之一。我们的结果还表明，亚致死性氧化损伤减少了活跃的和/或促进的运输，细胞内的铁可能是氧化应激和铁调节之间关系的关键因素。也许最有趣的是，我们揭示了氧化应激上调了所有F-、R-和S类脓毒素的转录，同时，一种细胞免疫蛋白(Pyocin S2免疫蛋白)下调，可能导致自杀活性。这一发现表明，腐殖素的产生可能是另一种新的防御宿主细胞氧化攻击的方案。
Pseudomonas aeruginosa, a pathogen infecting those with cystic fibrosis, encounters toxicity from phagocyte-derived reactive oxidants including hydrogen peroxide during active infection. P. aeruginosa responds with adaptive and protective strategies against these toxic species to effectively infect humans. Despite advances in our understanding of the responses to oxidative stress in many specific cases, the connectivity between targeted protective genes and the rest of cell metabolism remains obscure. Herein, we performed a genome-wide transcriptome analysis of the cellular responses to hydrogen peroxide in order to determine a more complete picture of how oxidative stress-induced genes are related and regulated. Our data reinforce the previous conclusion that DNA repair proteins and catalases may be among the most vital antioxidant defense systems of P. aeruginosa. Our results also suggest that sublethal oxidative damage reduces active and/or facilitated transport and that intracellular iron might be a key factor for a relationship between oxidative stress and iron regulation. Perhaps most intriguingly, we revealed that the transcription of all F-, R-, and S-type pyocins was upregulated by oxidative stress and at the same time, a cell immunity protein (pyocin S2 immunity protein) was downregulated, possibly leading to self-killing activity. This finding proposes that pyocin production might be another novel defensive scheme against oxidative attack by host cells.
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影响因子：
3.2
作者：
Hasset, DJ;Alsabbagh, E;Ochsner, UA
通讯作者：
Ochsner, UA
影响因子：
3.2
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BROWN, SM;HOWELL, ML;HASSETT, DJ
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HASSETT, DJ
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3.2
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Baysse, C;Meyer, JM;Cornelis, P
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Cornelis, P
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2.1
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de Almeida, DF
影响因子：
56.9
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Dervyn, E;Suski, C;Ehrlich, SD
通讯作者：
Ehrlich, SD