The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation.
The Arabidopsis NOT4A E3 ligase promotes PGR3 expression and regulates chloroplast translation.
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DOI：
10.1038/s41467-020-20506-4
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发表时间：
2021-01-11
影响因子：
16.6
通讯作者：
Gibbs DJ
中科院分区：
文献类型：
--
作者：
Bailey M;Ivanauskaite A;Grimmer J;Akintewe O;Payne AC;Osborne R;Labandera AM;Etherington RD;Rantala M;Baginsky S;Mulo P;Gibbs DJ
叶绿体功能需要核和叶绿体衍生蛋白的协调作用，包括数百个核编码的五肽重复序列（PPR）蛋白，调节质体mRNA代谢。尽管它们的数量和重要性，控制PPR表达的调控机制知之甚少。在这里，我们表明，拟南芥NOT4A泛素连接酶积极调节质子梯度调节3（PGR 3），翻译几个类囊体定位的光合作用成分和核糖体亚基叶绿体内所需的PPR蛋白的表达。NOT4A功能的丧失导致细胞色素b6f和NAD（P）H脱氢酶（NDH）复合物以及质体30 S核糖体的强烈消耗，这降低了mRNA翻译和光合能力，导致浅黄色和生长缓慢的表型。not4a突变体的定量转录组和蛋白质组分析显示，它缺乏PGR 3表达，其分子缺陷类似于PGR 3突变体。此外，我们表明，正常的质体功能恢复到not4a通过转基因PGR 3表达。我们的工作确定了NOT4A通过促进PGR 3的产生和叶绿体的翻译，对确保发育和胁迫响应过程中强大的光合功能至关重要。N0T4蛋白与核糖体结合，并且是酵母和动物中共翻译质量控制所需的。在此，Bailey等人表明拟南芥NOT4A正调控核编码的五肽重复（PPR）蛋白PGR 3的表达，并且是叶绿体中核糖体生物合成和mRNA翻译所需的。
Chloroplast function requires the coordinated action of nuclear- and chloroplast-derived proteins, including several hundred nuclear-encoded pentatricopeptide repeat (PPR) proteins that regulate plastid mRNA metabolism. Despite their large number and importance, regulatory mechanisms controlling PPR expression are poorly understood. Here we show that the Arabidopsis NOT4A ubiquitin-ligase positively regulates the expression of PROTON GRADIENT REGULATION 3 (PGR3), a PPR protein required for translating several thylakoid-localised photosynthetic components and ribosome subunits within chloroplasts. Loss of NOT4A function leads to a strong depletion of cytochrome b6f and NAD(P)H dehydrogenase (NDH) complexes, as well as plastid 30 S ribosomes, which reduces mRNA translation and photosynthetic capacity, causing pale-yellow and slow-growth phenotypes. Quantitative transcriptome and proteome analysis of the not4a mutant reveal it lacks PGR3 expression, and that its molecular defects resemble those of a pgr3 mutant. Furthermore, we show that normal plastid function is restored to not4a through transgenic PGR3 expression. Our work identifies NOT4A as crucial for ensuring robust photosynthetic function during development and stress-response, through promoting PGR3 production and chloroplast translation. NOT4 proteins associate with ribosomes and are required for co-translational quality control in yeast and animals. Here, Bailey et al. show that Arabidopsis NOT4A positively regulates the expression of the nuclear encoded pentatricopeptide repeat (PPR) protein PGR3 and is required for ribosome biogenesis and mRNA translation in the chloroplast.
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影响因子：
2.9
作者：
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通讯作者：
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