Dopaminergic nuclei in the chick midbrain express serotonin receptor subfamily genes.
Dopaminergic nuclei in the chick midbrain express serotonin receptor subfamily genes.
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DOI：
10.3389/fphys.2022.1030621
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发表时间：
2022
影响因子：
4
通讯作者：
Yamaguchi, Shinji
中科院分区：
文献类型：
--
作者：
Fujita, Toshiyuki;Aoki, Naoya;Mori, Chihiro;Serizawa, Shouta;Kihara-Negishi, Fumiko;Homma, Koichi J. J.;Yamaguchi, Shinji
关键词：
5-羟色胺（5-hydroxytryptamine，5-HT）是一种神经系统中保守的调节剂，参与神经功能的多个方面。中缝背核和正中核中的5-羟色胺能神经元为整个前脑和中脑提供上行神经支配。另一个重要的神经调节系统存在于中脑，多巴胺能系统，它与奖励处理和动机控制有关。多巴胺能神经元分布并聚集在脑中，经典地指定为A8-A16组。其中，与奖励加工和动机控制相关的A8-A10群位于中脑，并投射到前脑。最近，中脑多巴胺能神经元被多巴胺能神经元支配，并受到5-HT的调节，多巴胺能和多巴胺能系统之间的串扰引起了越来越多的关注。在鸟类中，先前的研究表明，中脑多巴胺能神经元位于A8-A10同源簇。然而，多巴胺能神经元的详细分布和多巴胺能和多巴胺能系统之间的串扰在鸟类知之甚少。为了提高对多巴胺能系统调节多巴胺能的理解，我们在鸡脑干中进行了原位杂交。我们制备了鸡多巴胺能神经元相关基因、酪氨酸羟化酶（TH）和多巴脱羧酶（DDC）、去甲肾上腺素相关基因、去甲肾上腺素转运蛋白（NAT）和多巴胺β-羟化酶（DBH）以及5-羟色胺受体基因的直系同源基因的RNA探针; 5-HTR1A、5-HTR1B、5-HTR1D、5-HTR1E、5-HTR1F、5-HTR2A、5-HTR2B、5-HTR2C、5-HTR3A、5-HTR4、5-HTR5A和5-HTR7。我们证实，酪氨酸羟化酶（TH）和NAT的表达是匹配的所有鸡多巴胺能核检查。这支持了NAT对多巴胺转运蛋白（DAT）功能的补偿是鸟类多巴胺能神经元的一种普遍特性。此外，我们发现5-HTR1A和5-HTR1B在中脑多巴胺能核中表达，这表明小鸡体内多巴胺能系统通过这些受体进行5-羟色胺调节。我们的发现将有助于我们在分子水平上了解鸟类多巴胺能和多巴胺能系统之间的相互作用。
Serotonin (5-hydroxytryptamine, 5-HT) is a phylogenetically conserved modulator of numerous aspects of neural functions. Serotonergic neurons in the dorsal and median raphe nucleus provide ascending innervation to the entire forebrain and midbrain. Another important neural modulatory system exists in the midbrain, the dopaminergic system, which is associated to reward processing and motivation control. Dopaminergic neurons are distributed and clustered in the brain, classically designated as groups A8–A16. Among them, groups A8–A10 associated with reward processing and motivation control are located in the midbrain and projected to the forebrain. Recently, midbrain dopaminergic neurons were shown to be innervated by serotonergic neurons and modulated by 5-HT, with the crosstalk between serotonergic and dopaminergic systems attracting increased attention. In birds, previous studies revealed that midbrain dopaminergic neurons are located in the A8-A10 homologous clusters. However, the detailed distribution of dopaminergic neurons and the crosstalk between serotonergic and dopaminergic systems in the bird are poorly understood. To improve the understanding of the regulation of the dopaminergic by the serotonergic system, we performed in situ hybridization in the chick brainstem. We prepared RNA probes for chick orthologues of dopaminergic neuron-related genes; tyrosine hydroxylase (TH) and dopa decarboxylase (DDC), noradrenaline related genes; noradrenaline transporter (NAT) and dopamine beta-hydroxylase (DBH), and serotonin receptor genes; 5-HTR1A, 5-HTR1B, 5-HTR1D, 5-HTR1E, 5-HTR1F, 5-HTR2A, 5-HTR2B, 5-HTR2C, 5-HTR3A, 5-HTR4, 5-HTR5A, and 5-HTR7. We confirmed that the expression of tyrosine hydroxylase (TH) and NAT was well matched in all chick dopaminergic nuclei examined. This supported that the compensation of the function of dopamine transporter (DAT) by NAT is a general property of avian dopaminergic neurons. Furthermore, we showed that 5-HTR1A and 5-HTR1B were expressed in midbrain dopaminergic nuclei, suggesting the serotonergic regulation of the dopaminergic system via these receptors in chicks. Our findings will help us understand the interactions between the dopaminergic and serotonergic systems in birds at the molecular level.
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影响因子：
2.9
作者：
Atoji, Yasuro;Wild, J. Martin
通讯作者：
Wild, J. Martin
影响因子：
4
作者：
Fujita T;Aoki N;Mori C;Fujita E;Matsushima T;Homma KJ;Yamaguchi S
通讯作者：
Yamaguchi S
影响因子：
4
作者：
Fujita, Toshiyuki;Aoki, Naoya;Mori, Chihiro;Fujita, Eiko;Matsushima, Toshiya;Homma, Koichi J.;Yamaguchi, Shinji
通讯作者：
Yamaguchi, Shinji
影响因子：
2.5
作者：
Bonnavion, Patricia;Bernard, Jean-Francois;Fabre, Veronique
通讯作者：
Fabre, Veronique
影响因子：
4.7
作者：
BRUINVELS, AT;LANDWEHRMEYER, B;PALACIOS, JM
通讯作者：
PALACIOS, JM